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| Product Name | ER Red Fluorescent Probe (Glibenclamide-Based, Live-Cell Imaging) |
| Catalog No. | RESK-0337 |
| Description | This cell membrane-permeable fluorescent probe is highly selective for the endoplasmic reticulum (ER) of live cells. Unlike the conventional dye DiOC6(3), it shows virtually no mitochondrial staining, labels the ER at low concentrations, and exhibits minimal cytotoxicity. After live-cell staining with the optimized protocol, aldehyde fixation can partially preserve the staining pattern. The probe is a conjugate of a red fluorophore and glibenclamide (glyburide), with Ex = 587 nm and Em = 615 nm. Glibenclamide is a widely used hypoglycemic drug and an important research tool for studying pancreatic beta cells, insulin secretion, cardiomyocyte function in heart failure, and arrhythmia. It binds with high affinity to the sulfonylurea receptor (SUR) of ATP-sensitive K+ (KATP) channels, which are enriched on the endoplasmic reticulum, making this conjugate well suited as an ER fluorescent probe. The product is supplied as a 1 mM stock solution in DMSO; the recommended working concentration is approximately 1 uM. |
| Intended Use | Selective red-fluorescent staining of the endoplasmic reticulum in live cells for fluorescence microscopy and subcellular imaging. For research use only. |
| Principle / Technology | Glibenclamide binds with high affinity to the sulfonylurea receptor (SUR) of ATP-sensitive K+ (KATP) channels, which are enriched on ER membranes. Conjugation of glibenclamide to a red fluorophore therefore targets the dye to the endoplasmic reticulum of live cells. |
| Detection Method | Fluorescence microscopy using a TRITC filter set; Ex/Em = 587/615 nm. |
| Sample Type | Live cultured cells (adherent or suspension) for subcellular organelle imaging. |
| Performance Range / Specifications | Formula: C44H42BClF2N6O7S2 Molecular weight: 915.23 Appearance: liquid Ex/Em: 587/615 nm Filter: TRITC Stock concentration: 1 mM in DMSO Recommended working concentration: ~1 uM |
| Specificity | Highly selective for the endoplasmic reticulum via glibenclamide binding to ER-enriched sulfonylurea receptors; virtually no mitochondrial staining at working concentrations. |
| Reaction Conditions / Protocol | Dilute the 1 mM DMSO stock into pre-warmed live-cell imaging medium to a final concentration of approximately 1 uM, incubate live cells under normal culture conditions, then wash and image. After live-cell staining, fixation with formaldehyde can partially retain the staining pattern. |
| Components / Formulation | 1 mM stock solution of the glibenclamide-based red fluorescent ER probe in DMSO. |
| Storage Conditions | Store at -20 °C or below, protected from light and kept dry. Avoid repeated freeze-thaw cycles. |
| Shelf Life | At least 6 months when stored as recommended. |
| Package Specifications | 20 uL (1 mM) |
| Product Form | Liquid (solution in DMSO) |
| Concentration | 1 mM stock solution in DMSO; recommended working concentration approximately 1 uM. |
| Molecular Weight | 915.23 |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for at least 6 months at -20 °C or below, protected from light and moisture; avoid repeated freeze-thaw cycles. |
| Compatibility | Compatible with TRITC filter sets; suitable for live-cell imaging and subsequent aldehyde fixation. For multicolor staining, sequential (not simultaneous) staining with other organelle probes is recommended. |
| Application Notes / Precautions | This probe primarily stains the ER of live cells; fixed samples are essentially not stained. Anti-fade mounting medium is not required for live-cell imaging. Fluorescence will gradually photobleach; complete detection within 1-2 h after staining. Fixation partially preserves the signal, but no quantitative data on fluorescence duration are available. Including Ca2+/Mg2+ in the staining buffer (e.g., HBSS) helps maintain cell condition and improves staining. For multicolor staining with other probes, stain sequentially rather than simultaneously. Avoid repeated freeze-thaw cycles. |
For research use only, not for clinical use.
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