- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | ER Blue-White Fluorescent Probe (DPX, Live-Cell Imaging) |
| Catalog No. | RESK-0335 |
| Description | This cell membrane-permeable fluorescent probe is highly selective for the endoplasmic reticulum (ER) of live cells. Unlike the conventional dye DiOC6(3), it shows virtually no mitochondrial staining, labels the ER at low concentrations, and exhibits minimal cytotoxicity. After live-cell staining with the optimized protocol, aldehyde fixation can partially preserve the staining pattern. The probe emits blue-white fluorescence with Ex = 374 nm and Em = 430-640 nm and belongs to the Dapoxyl (DPX) dye family, whose members feature long emission wavelengths, high extinction coefficients, high quantum yields, and large Stokes shifts. These dyes are environmentally sensitive: increasing polarity can shift the emission maximum to longer wavelengths and reduce the quantum yield. The product is supplied as a 1 mM stock solution in DMSO; the recommended working concentration is 100 nM-1 uM. |
| Intended Use | Selective fluorescent staining of the endoplasmic reticulum in live cells for fluorescence microscopy and subcellular imaging. For research use only. |
| Principle / Technology | A cell-permeable Dapoxyl (DPX)-family fluorophore that selectively accumulates in the endoplasmic reticulum of live cells, enabling ER-specific fluorescence labeling. |
| Detection Method | Fluorescence microscopy using a DAPI or UV longpass filter set; Ex/Em = 374/430-640 nm. |
| Sample Type | Live cultured cells (adherent or suspension) for subcellular organelle imaging. |
| Performance Range / Specifications | Formula: C26H21F5N4O4S Molecular weight: 580.53 Appearance: yellow solution Ex/Em: 374/430-640 nm Filter: DAPI or UV longpass Stock concentration: 1 mM in DMSO Recommended working concentration: 100 nM-1 uM |
| Specificity | Highly selective for the endoplasmic reticulum; virtually no mitochondrial staining at working concentrations. |
| Reaction Conditions / Protocol | Dilute the 1 mM DMSO stock into pre-warmed live-cell imaging medium to a final concentration of 100 nM-1 uM, incubate live cells under normal culture conditions, then wash and image. After live-cell staining, aldehyde fixation can partially retain the staining pattern. |
| Components / Formulation | 1 mM stock solution of the DPX-based ER probe in DMSO. |
| Storage Conditions | Store at -20 °C or below, protected from light and kept dry. Avoid repeated freeze-thaw cycles. |
| Shelf Life | 1 year when stored as recommended. |
| Package Specifications | 50 uL |
| Product Form | Liquid (yellow solution in DMSO) |
| Concentration | 1 mM stock solution in DMSO; recommended working concentration 100 nM-1 uM. |
| Molecular Weight | 580.53 |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for 1 year at -20 °C or below, protected from light and moisture; avoid repeated freeze-thaw cycles. |
| Compatibility | Compatible with DAPI or UV longpass filter sets; suitable for live-cell imaging and subsequent aldehyde fixation. |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles. The dye is environment-sensitive; polarity changes may shift the emission maximum and lower the quantum yield. Staining is performed on live cells; aldehyde fixation after staining only partially preserves the signal. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
