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| Product Name | Enzyme Activity Assay Diluent |
| Catalog No. | ATRD-0013 |
| Description | A buffered diluent containing enzyme-stabilizing additives for the dilution of purified enzymes, cell lysates, and tissue homogenates prior to kinetic and endpoint enzyme activity measurements. |
| Intended Use | Diluting enzyme preparations for measurement of specific activity using colorimetric, fluorometric, and luminometric enzyme assay kits without loss of catalytic function. |
| Principle / Technology | Glycerol and BSA stabilize protein conformation; DTT or beta-mercaptoethanol maintains reduced state of active site cysteine residues; EDTA chelates inhibitory heavy metals. |
| Detection Method | Enzyme activity linearity with dilution, specific activity comparison pre and post dilution |
| Sample Type | Purified recombinant enzymes, cell lysates, mitochondrial fractions, microsomal preparations, tissue homogenates |
| Performance Range / Specifications | Enzyme activity retention >95% for 2 hours on ice |
| Sensitivity / LOD | N/A |
| Specificity | Formulation optimized per enzyme class; reducing agent-free option for disulfide-containing enzymes |
| Reaction Conditions / Protocol | Dilute enzyme preparation to appropriate activity range for assay; keep on ice; use within 2 hours. |
| Components / Formulation | Tris-HCl or phosphate buffer pH 7.0–7.5, BSA 0.1%, glycerol 10%, DTT 1 mM, EDTA 0.1 mM |
| Storage Conditions | 2–8°C |
| Shelf Life | 6 months; verify reducing agent activity before use |
| Package Specifications | 50 mL, 100 mL |
| Product Form | Liquid, ready to use |
| Quality Control | Enzyme activity recovery validation with reference enzymes, reducing agent concentration verification |
| Key Features | Enzyme activity preserved during dilution; compatible with most enzyme assay kits; multiple buffer options available; glycerol-stabilized |
For research use only, not for clinical use.
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