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| Product Name | Enhanced First-Strand cDNA Synthesis Kit with gDNA Removal |
| Catalog No. | NATR-HMM-0177 |
| Description | An enhanced first-strand cDNA synthesis system designed for low-input, degraded and impurity-containing RNA, with rapid genomic DNA removal and flexible priming. |
| Intended Use | First-strand cDNA synthesis; reverse transcription; PCR; qPCR. |
| Principle / Technology | A high-efficiency reverse transcriptase and optimized buffer synthesize cDNA after rapid genomic DNA removal, using oligo(dT)20VN and random hexamer primers. |
| Sample Type | Total RNA, including low-input, degraded or impurity-containing RNA. |
| Performance Range / Specifications | 50 or 100 reactions at 20 µL per reaction; genomic DNA removal of up to 500 ng at 42°C for approximately 2 minutes; 9.9 kb cDNA demonstrated, with full-length synthesis capability up to 20 kb. |
| Specificity | The supplied oligo(dT)20VN and random hexamer primers support broad transcript coverage. |
| Reaction Conditions / Protocol | Remove genomic DNA at 42°C for approximately 2 minutes, then perform reverse transcription according to the recommended primer and temperature conditions. |
| Components / Formulation | Reverse transcriptase, optimized reaction buffer, genomic DNA removal module, oligo(dT)20VN primers and random hexamer primers. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 50 reactions (20 µL per reaction); 100 reactions (20 µL per reaction). |
| Product Form | First-strand cDNA synthesis kit with genomic DNA removal. |
| Quality Control | Evaluated with degraded RNA and RNA containing residual ethanol, isopropanol or water-saturated phenol. |
| Key Features | Strong impurity tolerance; suitable for low-input and degraded RNA; rapid genomic DNA removal; long-cDNA synthesis; flexible priming. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with downstream PCR and qPCR. |
| Application Notes / Precautions | Choose oligo(dT)20VN, random hexamers or a combination according to transcript structure and downstream amplification requirements. |
For research use only, not for clinical use.
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