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| Product Name | Endotoxin-Free Plasmid DNA Mini Extraction Kit |
| Catalog No. | DREK-0091 |
| Description | A spin-column kit for rapid purification of high-yield plasmid DNA with low endotoxin carryover. The workflow combines alkaline lysis, selective silica-membrane binding and dedicated impurity removal for transfection-ready DNA. |
| Intended Use | Plasmid DNA purification for restriction digestion, PCR, sequencing, ligation, transformation and cell transfection. |
| Principle / Technology | Optimized alkaline lysis followed by selective silica-membrane adsorption and endotoxin-removal chemistry. |
| Detection Method | Downstream DNA analysis and transfection performance. |
| Sample Type | 1-5 mL overnight bacterial culture. |
| Performance Range / Specifications | Approximately 25-minute workflow; plasmid DNA yield up to 50 µg; endotoxin residue ≤0.1 EU/µg DNA. |
| Specificity | Selective recovery of plasmid DNA while reducing endotoxin, protein, genomic DNA and other bacterial impurities. |
| Reaction Conditions / Protocol | Harvest the bacterial culture, resuspend and lyse the pellet, neutralize and clarify the lysate, bind plasmid DNA to the spin column, wash and elute. |
| Components / Formulation | Alkaline lysis, neutralization, binding, wash and elution reagents with silica spin columns and collection tubes. |
| Storage Conditions | Store at 15-25°C. |
| Package Specifications | 50 reactions. |
| Product Form | Endotoxin-free plasmid DNA spin-column kit. |
| Quality Control | Yield, DNA integrity, purity and endotoxin-removal performance are evaluated during release testing. |
| Key Features | Up to 50 µg yield; endotoxin ≤0.1 EU/µg; approximately 25-minute workflow; no hot-cold phase switching; transfection-compatible DNA. |
| Shipping Conditions | Ship at room temperature. |
| Compatibility | Common mammalian cell transfection and routine molecular biology workflows. |
| Application Notes / Precautions | Use a healthy overnight culture and avoid overloading the column. Handle lysates gently after alkaline lysis to minimize genomic DNA contamination. |
| Batch-to-Batch Consistency | Yield, purity and endotoxin-removal performance are controlled across production batches. |
For research use only, not for clinical use.
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