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| Product Name | ELISA Sample Diluent, Universal, Low-Endotoxin |
| Catalog No. | ATRD-0017 |
| Description | A universal sample diluent for ELISA applications, formulated with low endotoxin content for use with cell culture supernatant and recombinant protein samples where endotoxin contamination may confound biological readouts. The diluent contains a proprietary blend of blocking proteins and detergents to minimize non-specific antibody binding across a broad range of ELISA formats, including sandwich, competitive, and indirect configurations. |
| Intended Use | Universal dilution of samples and standards in ELISA procedures requiring low endotoxin background. |
| Principle / Technology | Protein-based blocking buffer with detergent to reduce hydrophobic non-specific interactions |
| Detection Method | Colorimetric ELISA detection at 450 nm |
| Sample Type | Cell culture supernatant, recombinant proteins, purified antibodies, serum, plasma |
| Performance Range / Specifications | Dilution linearity: R² ≥0.99 across 2-log dilution range for typical cytokine standards |
| Sensitivity / LOD | Endotoxin content ≤0.05 EU/mL |
| Specificity | Broad format compatibility; optimized blocking proteins minimize cross-reactivity with anti-mouse, anti-rabbit, and anti-human detection antibodies |
| Reaction Conditions / Protocol | Dilute samples and standards directly in this diluent; use as 50–100 µL per well in 96-well plate format |
| Components / Formulation | Phosphate-buffered saline with BSA (0.5%), casein hydrolysate, Tween 20 (0.05%), ProClin 300 preservative |
| Storage Conditions | 2–8 °C |
| Shelf Life | 18 months at 2–8 °C |
| Package Specifications | 200 mL |
| Product Form | Liquid, ready-to-use |
| Quality Control | Endotoxin measured by LAL assay per lot; lot-to-lot consistency verified by parallel standard curve analysis |
| Key Features | Low endotoxin formulation essential for cell-based assays where endotoxin can artifactually stimulate cytokine release |
| Purity | Endotoxin ≤0.5 EU/mL; sterile-filtered; protein and preservative content as specified |
| Concentration | Ready-to-use; no dilution required |
| Activity / Unit Definition | Dilution linearity R² ≥0.99 across specified range |
| Molecular Weight | Not applicable — buffered protein solution |
| Source / Origin | Bovine serum albumin, casein, and synthetic buffer components |
| pH Range / Optimal pH | pH 7.2–7.6 |
| Shipping Conditions | Ambient temperature with cold packs for summer months |
| Expiration Date / Stability | 12–18 months at 2–8 °C |
| Regulatory / Compliance | For IVD use; manufactured under ISO 13485 quality system |
| Compatibility | Compatible with ELISA, CLIA, and immunoturbidimetric assay platforms |
| Recommended Buffer System | PBS-based with protein stabilizers and antimicrobial preservative |
| Application Notes / Precautions | Bring to room temperature before use; avoid repeated freeze-thaw; use aseptic technique for opened bottles |
| Batch-to-Batch Consistency | Dilution recovery 90–110% for reference analyte panel per lot |
For research use only, not for clinical use.
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