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| Product Name | dNTP Mix (10 mM Each), PCR Grade |
| Catalog No. | NATR-HMM-0126 |
| Description | A high-purity deoxynucleotide triphosphate mixture containing dATP, dCTP, dGTP, and dTTP each at a concentration of 10 mM in nuclease-free water. The dNTPs are prepared from highly purified nucleotide stocks and tested for functional performance in PCR and other DNA polymerase-based applications. |
| Intended Use | Substrate for DNA polymerases in PCR, qPCR, reverse transcription, cDNA synthesis, DNA sequencing, nick translation, random priming, end-filling, and other DNA synthesis reactions in molecular biology research. |
| Principle / Technology | DNA polymerases catalyze the sequential addition of dNTPs to the 3ʹ-OH terminus of a nascent DNA strand, using the template strand as a guide for Watson-Crick base pairing. The dNTPs serve as the monomeric building blocks for DNA polymerization, with the phosphodiester bond formation releasing pyrophosphate as a by-product. |
| Detection Method | PCR amplification efficiency and fidelity testing; HPLC purity analysis; functional testing in DNA sequencing; spectrophotometric verification of nucleotide concentration at specific wavelengths |
| Sample Type | Compatible with all DNA-dependent DNA polymerases including Taq, Pfu, Phusion, Q5, KOD, and Vent; suitable for both standard and high-fidelity PCR, cDNA synthesis, and sequencing |
| Performance Range / Specifications | Concentration: 10 mM each dNTP (40 mM total nucleotide); HPLC purity: ≥99% for each nucleotide; PCR performance: ≥95% amplification efficiency across a 6-log template dilution; pH: 7.0–7.5 at 25°C; DNase/RNase: none detected |
| Sensitivity / LOD | Effective nucleotide concentration supports robust amplification from as little as 1 pg of plasmid template in a 50 μL PCR reaction; no detectable PCR inhibition when dNTPs constitute up to 50% of reaction volume at working concentration |
| Specificity | HPLC-verified nucleotide purity ≥99% ensures minimal incorporation errors; each dNTP species validated at equivalent concentrations (≤5% variation between dNTPs) for balanced PCR amplification |
| Reaction Conditions / Protocol | Thaw on ice, mix gently by vortex, centrifuge briefly before opening; typical final concentration in PCR: 0.2 mM each dNTP (1:50 dilution of 10 mM stock); for long-range PCR or sequencing, adjust to 0.3–0.5 mM each; aliquot into single-use volumes to maintain quality |
| Components / Formulation | dATP, dCTP, dGTP, dTTP (sodium salts), each dissolved at 10 mM in nuclease-free, deionized water (≥18.2 MΩ·cm), pH adjusted to 7.0–7.5 with NaOH; supplied as a pre-mixed solution or as four individual 100 mM stock solutions for customized mixing |
| Storage Conditions | –20°C for long-term storage; stable at 2–8°C for up to 1 month; avoid alkaline pH and repeated freeze-thaw cycles (≤10); aliquot to minimize freeze-thaw frequency |
| Shelf Life | 24 months at –20°C from date of manufacture |
| Package Specifications | 0.5 mL (5 μmol each dNTP), 1 mL (10 μmol each), 5 mL, and 25 mL bulk options |
| Product Form | Neutralized, ready-to-use liquid solution (sodium salts) |
| Quality Control | Each lot tested: HPLC purity ≥99% per nucleotide; PCR amplification efficiency 95–105% with Taq polymerase and standard primers; DNA sequencing read length >800 bases (Phred Q20); no detectable DNase/RNase activity; endotoxin <0.01 EU/μmol |
| Key Features | Ultra-pure HPLC-verified nucleotides ensure reproducible PCR; pre-mixed format eliminates individual nucleotide addition errors; ≥99% purity minimizes sequencing background; validated for next-generation sequencing library preparation; sodium salt formulation at neutral pH ensures long-term stability and enzyme compatibility |
For research use only, not for clinical use.
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