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| Product Name | DNA Gel Loading Dye, 6×, for Agarose Gels, with Tracking Dyes |
| Catalog No. | NATR-HMM-0150 |
| Description | 6× concentrated DNA gel loading dye containing bromophenol blue and xylene cyanol FF as dual tracking dyes for monitoring electrophoresis migration in agarose gels. The dense sucrose/glycerol base ensures samples sink evenly into gel wells without floating or diffusing. Bromophenol blue co-migrates with ~300 bp DNA fragments while xylene cyanol FF migrates at ~4,000 bp position in 1% agarose, providing reference points for gel running time. |
| Intended Use | Loading of DNA samples onto agarose gels for electrophoresis; visual tracking of electrophoresis progress; estimation of DNA fragment sizes by dye migration; sample density enhancement for well loading. |
| Principle / Technology | Dense sucrose/glycerol matrix (density >1.05 g/mL) causes samples to sink uniformly into wells; bromophenol blue (dark blue) migrates at ~300 bp in 1% agarose; xylene cyanol FF (light blue-green) migrates at ~4,000 bp in 1% agarose; dyes allow real-time monitoring of electrophoresis progress |
| Detection Method | Mix 1 volume loading dye with 5 volumes DNA sample (e.g., 2 µL dye + 10 µL sample); load directly into agarose gel wells; run gel at 5-10 V/cm until bromophenol blue reaches 2/3 to 3/4 of gel length |
| Sample Type | DNA samples in water, TE buffer, or reaction buffers; compatible with PCR products, restriction digests, plasmid DNA, genomic DNA; 10 µL-50 µL sample volume typical |
| Sensitivity / LOD | Dyes visible at 1× concentration during electrophoresis; no interference with DNA visualization by ethidium bromide, SYBR Safe, GelRed, or other DNA stains; dyes do not bind DNA or alter migration |
| Specificity | Inert tracking dyes — no interaction with DNA or agarose matrix; sucrose/glycerol matrix does not affect DNA migration; dual dyes cover both high and low MW range markers |
| Reaction Conditions / Protocol | Direct mixing — no incubation required; load immediately after mixing; store unused dye at 4 °C or RT |
| Components / Formulation | Bromophenol blue (0.05% w/v), xylene cyanol FF (0.05% w/v), sucrose (40% w/v) or glycerol (30% v/v), EDTA (10 mM, pH 8.0), Tris buffer (10 mM, pH 7.6), ultrapure water; 0.22 µm filtered |
| Storage Conditions | 4 °C for long-term; RT for up to 6 months; protect from light; do not freeze |
| Shelf Life | 36 months at 4 °C; 6 months at RT |
| Package Specifications | 1 mL, 5 mL, 10 mL, 25 mL in amber dropper bottles |
| Product Form | Dark blue viscous liquid, ready-to-use |
| Key Features | Dual tracking dyes for high and low MW reference; dense formulation ensures clean well loading; compatible with all common DNA stains; pre-mixed and ready-to-use; RNase/DNase-free; amber bottle for light protection; dropper cap for convenient dispensing |
| Purity | All dyes certified for electrophoresis; DNase/RNase tested free; heavy metals <1 ppm; no detectable DNA contamination |
| Concentration | 6× concentrate; use at 1× final concentration (1:6 dilution with sample) |
| Activity / Unit Definition | Polymerase activity defined as nmol dNTP incorporated per 30 min per unit; other activities per product specification |
| Molecular Weight | As specified per DNA markers or protein components |
| Source / Origin | Recombinant enzymes expressed in E. coli; synthetic oligonucleotides; ultrapure reagents |
| pH Range / Optimal pH | pH 7.6 ± 0.1 at 25 °C |
| Shipping Conditions | Ambient temperature; dark bottle for light protection |
| Expiration Date / Stability | 36 months at 4 °C; 6 months at RT; discard if dye color fades significantly (indicating photodegradation) or microbial growth observed; if dye precipitates, warm to 37 °C and vortex — precipitation does NOT indicate degradation |
| Regulatory / Compliance | For laboratory and research use only; RUO; not classified as dangerous goods; ISO 9001 |
| Compatibility | Compatible with all agarose gel concentrations (0.5-3%) and all common electrophoresis buffers (TAE, TBE, SB); EDTA content may shift migration of some DNA fragments at very high dye-to-sample ratios; dyes may interfere with some downstream enzymatic reactions (sequencing, cloning) at high carryover — purify DNA after gel extraction |
| Recommended Buffer System | Tris-HCl, KCl, MgCl₂-based reaction buffer; TE for nucleic acid storage |
| Application Notes / Precautions | Use dedicated PCR workspace; aliquot enzymes to avoid contamination; monitor no-template controls |
| Batch-to-Batch Consistency | Enzyme activity within ±15% of reference lot; PCR performance verified with control template |
For research use only, not for clinical use.
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