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| Product Name | DNA Gel Extraction and Purification Kit |
| Catalog No. | NATR-HMM-0119 |
| Description | A silica membrane spin column kit for the rapid extraction and purification of DNA fragments from agarose gels following electrophoretic separation. The kit effectively recovers DNA fragments ranging from 70 bp to 10 kb with high efficiency and purity suitable for downstream enzymatic reactions. |
| Intended Use | Recovery of DNA fragments from agarose gels after restriction digestion, PCR product purification, or other electrophoretic separations for cloning, sequencing, labeling, and in vitro transcription applications. |
| Principle / Technology | Agarose gel slices are dissolved in a chaotropic salt buffer at 50–60°C. The released DNA binds selectively to a silica membrane in the presence of high chaotropic salt concentrations. Contaminants including agarose, ethidium bromide, salts, and enzymes are removed by washing with ethanol-based buffer. Purified DNA is eluted in a small volume of buffer or water. |
| Detection Method | UV spectrophotometry for recovery yield and purity; agarose gel electrophoresis for size verification; restriction digestion and ligation efficiency for functionality assessment |
| Sample Type | DNA fragments in standard (1–3%) agarose gels prepared with TAE or TBE buffer; both low-melting-point and standard agarose compatible |
| Performance Range / Specifications | Fragment size range: 70 bp to 10 kb; recovery efficiency: 60–90% (size-dependent); elution volume range: 10–50 μL; typical DNA purity: A260/A280 ratio 1.75–1.85 |
| Sensitivity / LOD | Recovers as little as 25 ng of DNA from agarose gel slices visible by ethidium bromide or SYBR Safe staining |
| Specificity | No detectable agarose carryover; removal of >99% of gel contaminants; purified DNA free of agarose digestion by-products that inhibit ligation |
| Reaction Conditions / Protocol | Excise DNA band from gel under UV illumination (minimize UV exposure), weigh gel slice, add 3 volumes binding buffer per volume of gel, incubate at 50–60°C for 10 minutes (vortex every 2–3 minutes) until dissolved, add 1 volume isopropanol, transfer to spin column, centrifuge 1 minute, wash with 700 μL wash buffer, centrifuge 1 minute, wash with 500 μL additional wash buffer, centrifuge dry 2 minutes, elute with 10–50 μL elution buffer or water, incubate 1 minute, centrifuge 1 minute |
| Components / Formulation | Binding buffer (sodium iodide or guanidine hydrochloride, Tris-HCl), wash buffer concentrate (Tris-HCl, NaCl, EDTA, ethanol), elution buffer (10 mM Tris-HCl pH 8.5), silica membrane spin columns with collection tubes |
| Storage Conditions | All buffers and columns stored at room temperature (15–25°C); protect binding buffer from light; tightly close binding buffer bottle to prevent oxidation |
| Shelf Life | 24 months from date of manufacture at recommended storage |
| Package Specifications | 50 and 250 preparations; all columns, tubes, and buffers included |
| Product Form | Liquid buffers; silica membrane spin columns |
| Quality Control | Each lot tested: recovery of 500 bp fragment from 1% agarose: >80% recovery efficiency; recovered DNA functionally validated by T4 DNA ligase ligation efficiency >90% for cohesive-end cloning; absence of nucleases in recovered DNA; sequencing quality verified |
| Key Features | Efficient recovery across a broad fragment size range; compatible with TAE and TBE buffer gels; minimal UV-induced DNA damage protocol guidelines; broadest size recovery range among gel extraction methods; sodium iodide-based binding buffer dissolves gel rapidly |
For research use only, not for clinical use.
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