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| Product Name | Direct PCR Kit for Blood and Tissue (No DNA Extraction Required) |
| Catalog No. | NATR-HMM-0148 |
| Description | A polymerase chain reaction kit engineered to amplify DNA directly from crude blood, tissue, and other biological specimens without prior DNA extraction or purification steps. The proprietary polymerase formulation and buffer system resist common PCR inhibitors found in biological samples including heme, hemoglobin, and tissue debris. This direct amplification approach dramatically reduces hands-on time and eliminates the risk of sample loss during purification. |
| Intended Use | Direct amplification of target DNA sequences from unpurified biological samples, bypassing conventional DNA extraction steps. |
| Principle / Technology | Direct PCR with inhibitor-tolerant DNA polymerase |
| Detection Method | Gel electrophoresis or real-time fluorescence detection (requires separate dye addition for real-time format) |
| Sample Type | Whole blood (EDTA or citrate), blood spots on FTA cards, fresh or frozen tissue biopsies, buccal swabs, hair follicles, plant leaf punches |
| Performance Range / Specifications | Reliable amplification from up to 20% whole blood in final reaction volume; amplicon size up to 4 kb from blood |
| Sensitivity / LOD | Detection from as little as 0.5 µL whole blood per 25 µL reaction |
| Specificity | Primer-dependent specificity; hot-start antibody modification ensures no non-specific amplification |
| Reaction Conditions / Protocol | Add 0.5–5 µL crude sample to 25 µL reaction mix; initial denaturation: 95 °C for 5 min; 35–40 cycles of (95 °C 30 sec, 55–65 °C 30 sec, 72 °C 60 sec per kb); final extension: 72 °C 5 min |
| Components / Formulation | 2× Direct PCR Master Mix (containing hot-start DNA polymerase, dNTPs, MgCl₂, and buffer), PCR-grade water, protocol optimization guide |
| Storage Conditions | –20 °C; stable at 4 °C for up to 4 weeks |
| Shelf Life | 12 months at –20 °C |
| Package Specifications | 200 reactions (2.5 mL of 2× master mix) |
| Product Form | Liquid 2× master mix in glycerol-containing buffer |
| Quality Control | Each lot validated by direct amplification from 10 human whole blood samples and 10 mouse tail biopsies; amplification success rate ≥95% |
| Key Features | Raw-sample-to-PCR workflow eliminates DNA purification entirely, cutting total processing time by over 60% |
| Purity | DNase/RNase free; PCR inhibitor free; A260/A280 ≥1.8 for nucleic acid products |
| Concentration | As specified on product label; enzyme units per µL as stated |
| Activity / Unit Definition | Polymerase activity defined as nmol dNTP incorporated per 30 min per unit; other activities per product specification |
| Molecular Weight | As specified per DNA markers or protein components |
| Source / Origin | Recombinant enzymes expressed in E. coli; synthetic oligonucleotides; ultrapure reagents |
| pH Range / Optimal pH | pH 7.5–8.5 for PCR; pH 8.0 for most molecular biology applications |
| Shipping Conditions | Cold packs or dry ice for enzymes; ambient for buffers and DNA markers |
| Expiration Date / Stability | 12–24 months at –20 °C for enzymes; 12–24 months at room temperature for buffers |
| Regulatory / Compliance | Research use; ISO 13485 manufacturing for select products |
| Compatibility | Compatible with standard thermal cyclers, real-time PCR instruments, and gel electrophoresis systems |
| Recommended Buffer System | Tris-HCl, KCl, MgCl₂-based reaction buffer; TE for nucleic acid storage |
| Application Notes / Precautions | Use dedicated PCR workspace; aliquot enzymes to avoid contamination; monitor no-template controls |
| Batch-to-Batch Consistency | Enzyme activity within ±15% of reference lot; PCR performance verified with control template |
For research use only, not for clinical use.
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