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| Product Name | Direct Cell-to-cDNA Synthesis Kit |
| Catalog No. | NATR-HMM-0202 |
| Description | An integrated cell-lysis and reverse-transcription kit that converts cultured cells directly to qPCR-ready first-strand cDNA without conventional RNA purification. |
| Intended Use | High-throughput cell-based drug screening; gene-expression analysis; preparation of first-strand cDNA directly from cultured cells. |
| Principle / Technology | Cells are lysed in culture plates with genomic-DNA removal, and the resulting RNA-containing lysate is converted directly to first-strand cDNA. |
| Sample Type | 10 to 10⁶ cultured adherent or suspension cells. |
| Performance Range / Specifications | Cell lysis and genomic-DNA removal in 7–9 minutes, followed by a 20-minute reverse-transcription step; total workflow approximately 27 minutes. |
| Reaction Conditions / Protocol | Lyse cells directly in the culture plate, add stop buffer, transfer or use the lysate in the reverse-transcription module, and synthesize first-strand cDNA for downstream qPCR. |
| Components / Formulation | Direct cell-lysis/genomic-DNA removal module and first-strand cDNA synthesis module. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 500 reactions. |
| Product Form | Integrated direct cell-to-cDNA kit. |
| Key Features | Approximately 27-minute workflow; no RNA purification; broad cell-type and cell-number compatibility; supports in-plate and high-throughput processing. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Generated cDNA is compatible with dye-based and probe-based qPCR assays. |
| Application Notes / Precautions | Process lysates promptly to minimize RNA degradation. Avoid excessive cell input and ensure thorough mixing with both lysis and stop reagents. |
For research use only, not for clinical use.
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