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| Product Name | Direct Cell One-Step SYBR RT-qPCR Kit |
| Catalog No. | NATR-HMM-0206 |
| Description | An extraction-free one-step dye-based RT-qPCR system for direct gene-expression analysis from cultured cells. |
| Intended Use | Rapid high-throughput cell-based drug screening and cellular gene-expression analysis. |
| Principle / Technology | Cells are lysed directly in culture plates with genomic-DNA removal, and the RNA-containing lysate is analyzed in a single dye-based reverse-transcription qPCR reaction. |
| Detection Method | SYBR Green dye-based one-step RT-qPCR. |
| Sample Type | 10 to 10⁶ cultured adherent or suspension cells. |
| Performance Range / Specifications | Complete cell-to-result workflow in approximately 52 minutes; compatible with 384-, 96-, 24-, 12- and 6-well cell-culture plates. |
| Sensitivity / LOD | Broad cell-input compatibility supports analysis from low to high cell numbers. |
| Specificity | Specialized additives suppress primer-dimer formation and improve quantitative specificity. |
| Reaction Conditions / Protocol | Perform 7–9 minute in-plate lysis and genomic-DNA removal, then add lysate directly to the one-step dye-based RT-qPCR reaction. |
| Components / Formulation | Cell-lysis/genomic-DNA removal module and one-step SYBR RT-qPCR master mix. |
| Storage Conditions | Store at −30 to −15°C. |
| Package Specifications | 100 reactions; 500 reactions. |
| Product Form | Complete direct-cell one-step dye-based RT-qPCR kit. |
| Quality Control | Cell-input compatibility, cell-type compatibility, primer-dimer suppression and dye-based amplification performance are evaluated. |
| Key Features | Approximately 52-minute workflow; extraction-free sample preparation; broad cell compatibility; high-throughput plate support; improved primer-dimer suppression. |
| Shipping Conditions | Ship at or below 0°C. |
| Compatibility | Compatible with common dye-based real-time PCR instruments and multiple cell-culture plate formats. |
| Application Notes / Precautions | Process fresh cells promptly, mix lysis and stop reagents thoroughly, and avoid excessive cell inputs that may inhibit amplification. |
For research use only, not for clinical use.
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