- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | DiR Iodide (DiIC18(7)) Cell Membrane Deep Red Fluorescent Probe |
| Catalog No. | RESK-0555 |
| Description | DiI, DiO, DiD, and DiR are a series of lipophilic fluorescent dyes used to stain cell membranes and other lipid-soluble biological structures. These environment-sensitive dyes are only weakly fluorescent before entering the cell membrane, and their fluorescence intensity is greatly enhanced upon binding to the membrane. They feature high extinction coefficients, polarity-dependent fluorescence, and short excited-state lifetimes. Once incorporated, the dye diffuses throughout the entire cell membrane and, at optimal concentration, stains the whole membrane. The different emission colors of the series (DiI: orange; DiO: green; DiD: red; DiR: deep red) facilitate live-cell multicolor labeling and flow cytometric analysis: DiO and DiI can be detected with standard FITC and TRITC filter sets, respectively; DiD, a red-shifted derivative of DiI, is especially suitable for labeling cells or tissues with significant autofluorescence; and DiR exhibits strong near-infrared penetration of cells and tissues with low autofluorescence in the near-infrared region, making it particularly well suited for in vivo imaging and tracing experiments. |
| Intended Use | Fluorescent labeling of cell membranes and other lipid-soluble biological structures, including live-cell multicolor labeling, flow cytometric analysis, and in vivo imaging or tracing experiments. |
| Principle / Technology | DiR Iodide is an environment-sensitive lipophilic carbocyanine dye. It is almost non-fluorescent in aqueous environments but becomes strongly fluorescent when inserted into the lipid bilayer of cell membranes. After incorporation, it diffuses laterally across the entire membrane, enabling uniform whole-membrane staining at optimal concentration. |
| Detection Method | Near-infrared fluorescence detection (Ex/Em 748 nm/780 nm); recommended optical filters: XF112-Omega or 41009-Chroma. |
| Sample Type | Animal and plant cells and tissues (no cell-type specificity), as well as other lipid-containing biological structures. |
| Performance Range / Specifications | English synonym: 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyaine iodide CAS No.: N/A Molecular formula: C63H101IN2 Molecular weight: 1013.4 Ex/Em: 748 nm/780 nm Recommended optical filters: XF112-Omega, 41009-Chroma Solubility: soluble in DMSO and ethanol |
| Specificity | No cell-type specificity: as a lipophilic dye, it labels animal and plant cells by inserting into lipid membranes and being excited for observation. DiR provides strong near-infrared penetration through cells and tissues and low near-infrared autofluorescence, making it especially suitable for in vivo imaging and tracing. |
| Reaction Conditions / Protocol | Minimize the time between probe loading and instrument measurement (excluding any stimulation period) to reduce potential errors. If the fluorescent color appears faint, increase the staining time and/or dye concentration. For HPLC analysis of this probe, use a normal-phase column with chloroform/methanol (~10:1). |
| Components / Formulation | DiR Iodide (DiIC18(7)) lipophilic carbocyanine dye, supplied as a single 25 mg unit. |
| Storage Conditions | Store at -20 °C, dry and protected from light. |
| Shelf Life | One year when stored at -20 °C, dry and protected from light. |
| Package Specifications | 25 mg |
| Molecular Weight | 1013.4 |
| Shipping Conditions | Shipped on wet ice. |
| Expiration Date / Stability | Valid for one year when stored at -20 °C, dry and protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Can be combined with other dyes of the same series (DiI, DiO, DiD) for live-cell multicolor labeling and flow cytometric analysis. |
| Recommended Buffer System | Soluble in DMSO and ethanol; dissolve in DMSO or ethanol to prepare stock solutions. |
| Application Notes / Precautions | Fluorescent probes of this type have some toxicity to live cells; complete detection and observation as soon as possible after staining. To observe morphological changes of living cells over several hours, lipophilic carbocyanine dyes such as DiI, DiO, DiD, or DiR are commonly used. If the staining signal is weak, try increasing the staining time and concentration. Shorten the interval between probe loading and measurement as much as possible (except for required stimulation time). |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
