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| Product Name | DiI (DiIC18(3)) Cell Membrane Orange-Red Fluorescent Probe |
| Catalog No. | RESK-0535 |
| Description | DiI (DiIC18(3)) is a lipophilic fluorescent dye used to stain cell membranes and other lipid-soluble biological structures. After entering the cell membrane, DiI diffuses across the entire membrane and, at optimal concentrations, stains the whole cell membrane. DiI is only weakly fluorescent before entering the membrane, but its fluorescence intensity is greatly enhanced upon membrane binding; upon excitation it emits orange-red fluorescence with a high extinction coefficient, moderate quantum yield, and a short excited-state lifetime. It can be detected with a standard TRITC filter set. DiI generally does not markedly affect cell viability and is therefore widely used as an anterograde or retrograde tracer and long-term tracer for living or fixed neural cells and tissues. Beyond simple membrane fluorescent labeling, it can also be used to monitor cell fusion and adhesion, track cell migration during development or transplantation, measure lipid diffusion within the membrane by FRAP (fluorescence recovery after photobleaching), assess cytotoxicity, and label lipoproteins. |
| Intended Use | Fluorescent labeling of cell membranes and other lipophilic biological structures; anterograde/retrograde and long-term tracing of living or fixed cells and tissues (e.g., neural cells); cell fusion and adhesion assays; cell migration tracking during development or transplantation; FRAP-based measurement of lipid diffusion in membranes; cytotoxicity testing; lipoprotein labeling. For research use only. |
| Principle / Technology | DiI is a lipophilic carbocyanine dye that inserts into lipid bilayers. It is virtually non-fluorescent before membrane incorporation but becomes strongly fluorescent once bound to the cell membrane, where it diffuses laterally to label the entire membrane at optimal concentration, producing orange-red fluorescence upon excitation. |
| Detection Method | Fluorescence detection using a standard TRITC filter set. Recommended filters: XF32-Omega, 31002-Chroma. Ex/Em = 550/567 nm. |
| Sample Type | Living or fixed cells and tissues of animal or plant origin (plant cells or tissues are generally prepared as protoplasts before staining); cell membranes and other lipid-containing membrane structures; lipoproteins. Bacterial cell membranes can theoretically be stained after removal of the cell wall. Exosomes possess membrane structures and can theoretically be stained, but staining performance has not been tested; please refer to published literature. |
| Performance Range / Specifications | Synonyms: DiIC18(3); DiI perchlorate; 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate CAS NO.: 41085-99-8 Molecular Formula: C59H97ClN2O4 Molecular Weight: 933.87 Appearance: Red to dark red, purple to deep purple powder Ex/Em: 550/567 nm Recommended filters: XF32-Omega, 31002-Chroma Purity: >=98% (TLC) Solubility: Soluble in DMF, DMSO, and ethanol |
| Specificity | No cell-type or species specificity. These lipophilic dyes are inserted into lipid membranes and observed after laser excitation; they show no specificity toward animal or plant cells. |
| Reaction Conditions / Protocol | If granular particles appear when DiI is diluted with sterile PBS, warm or sonicate the diluted solution for a period of time, or add a small amount of DMSO to promote dissolution. |
| Components / Formulation | Supplied as a red to dark red, purple to deep purple powder (lipophilic carbocyanine dye, DiI perchlorate). |
| Storage Conditions | Store at 4 °C, dry and protected from light. |
| Shelf Life | 1 year when stored at 4 °C, dry and protected from light. |
| Package Specifications | 10 mg |
| Product Form | Powder |
| Quality Control | Purity >=98% (TLC). |
| Purity | >=98% (TLC) |
| Molecular Weight | 933.87 |
| Shipping Conditions | Shipped with wet ice. |
| Expiration Date / Stability | Valid for 1 year when stored at 4 °C, dry and protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Detectable with standard TRITC filter sets. |
| Application Notes / Precautions | If particles appear in solutions diluted with sterile PBS, warm or sonicate the solution, or add a small amount of DMSO to aid dissolution. For bacterial membrane staining, remove the bacterial cell wall before staining. For plant cells or tissues, protoplasts are generally prepared before staining. Exosome staining is theoretically feasible but has not been tested; consult published literature. Protect from light during storage. |
For research use only, not for clinical use.
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