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| Product Name | DAPI Staining Solution, 5 mg/mL |
| Catalog No. | RERE-0304 |
| Description | Highly concentrated DAPI stock at 5 mg/mL, several hundred times the typical 0.5 to 10 µg/mL working range, so a single 1 mL aliquot supplies many staining rounds. DAPI binds double stranded DNA strongly, crosses intact cell membranes of both live and fixed cells, and shows blue fluorescence with excitation at 360 nm and emission at 460 nm in the DNA-bound state, at low toxicity to living cells. Common applications include apoptosis observation, general nuclear counterstaining and double stranded DNA labeling. Store at -20 °C away from light; shelf life is 12 months. |
| Intended Use | Stock solution for nuclear staining, apoptosis observation and cell labeling workflows |
| Principle / Technology | DAPI intercalates preferentially with double stranded DNA, and the bound complex fluoresces blue under near-UV excitation |
| Specificity | 5 mg/mL stock; working range 0.5-10 µg/mL; excitation 360 nm and emission 460 nm when DNA bound; 12 month shelf life |
| Reaction Conditions / Protocol | Dilute the stock into the working concentration, incubate with cells, wash and image with a fluorescence microscope or flow cytometer |
| Storage Conditions | -20 °C, protected from light |
| Package Specifications | 1 mL |
| Key Features | Concentrated stock reduces per-experiment cost; membrane permeant for live and fixed cells; 12 month shelf life |
For research use only, not for clinical use.
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