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| Product Name | DAPI Staining Solution, 5 µg/mL, with Mounting Medium |
| Catalog No. | RERE-0279 |
| Description | Fluorescent nuclear stain built around DAPI at 5 µg/mL and packaged together with its mounting medium for a complete staining-to-imaging workflow. DAPI crosses intact cell membranes, so both live and fixed cells can be stained, and the recommended working range is 0.5 to 10 µg/mL. Bound to double stranded DNA the complex excites at 360 nm and emits at 460 nm, giving bright blue nuclei with little toxicity to living cells. Store at -20 °C away from light; shelf life is 6 months. |
| Intended Use | Nuclear counterstaining, apoptosis observation and cell labeling in live or fixed preparations |
| Principle / Technology | DAPI intercalates preferentially with double stranded DNA, and the bound dye fluoresces blue when excited near 360 nm |
| Specificity | 5 µg/mL DAPI with mounting medium; working range 0.5-10 µg/mL; excitation 360 nm and emission 460 nm when DNA bound; 6 month shelf life |
| Reaction Conditions / Protocol | Incubate the cells with the diluted DAPI solution, wash, apply the supplied mounting medium and image with a fluorescence microscope |
| Storage Conditions | -20 °C, protected from light |
| Package Specifications | 10 mL |
| Key Features | Membrane permeant stain for live and fixed cells; mounting medium included; low toxicity to living cells; 6 month shelf life |
For research use only, not for clinical use.
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