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| Product Name | DAPI Staining Solution, 5 µg/mL |
| Catalog No. | RERE-0237 |
| Description | Ready-to-use DAPI solution at 5 µg/mL for fluorescent nuclear staining. The dye binds double-stranded DNA strongly and emits blue fluorescence, so nuclei can be visualised on a fluorescence microscope or counted by flow cytometry. Because it penetrates intact membranes it stains both live and fixed cells, and it is a routine companion stain in apoptosis assays. The working range is normally 0.5 to 10 µg/mL. Store at -20 °C away from light; the solution keeps for 6 months. |
| Intended Use | Fluorescent nuclear counterstaining of live and fixed cells, including apoptosis assays |
| Principle / Technology | DAPI intercalates into double-stranded DNA and fluoresces blue upon ultraviolet excitation |
| Specificity | 5 µg/mL; ready-to-use solution; free dye excites at 340 nm and emits at 488 nm, shifting to 360 nm and 460 nm when bound to double-stranded DNA; 6 month shelf life |
| Reaction Conditions / Protocol | Apply the solution to the sample, incubate briefly, wash, then examine under a fluorescence microscope or by flow cytometry |
| Storage Conditions | -20 °C, protected from light |
| Package Specifications | 10 mL | 50 mL |
| Key Features | Ready-to-use concentration; compatible with live and fixed cells; 6 month shelf life |
For research use only, not for clinical use.
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