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| Product Name | DAPI Staining Solution, 10 µg/mL |
| Catalog No. | RERE-0238 |
| Description | DAPI supplied at 10 µg/mL as a ready-to-use nuclear stain. On binding double-stranded DNA the dye gives a bright blue signal, allowing nuclei to be located on a fluorescence microscope or enumerated by flow cytometry. It crosses intact membranes, so it works with live and fixed preparations alike and is often paired with annexin or other apoptotic markers. Recommended working concentrations span 0.5 to 10 µg/mL. Store at -20 °C away from light; the solution keeps for 6 months. |
| Intended Use | Fluorescent nuclear counterstaining of live and fixed cells, including apoptosis assays |
| Principle / Technology | DAPI intercalates into double-stranded DNA and fluoresces blue upon ultraviolet excitation |
| Specificity | 10 µg/mL; ready-to-use solution; free dye excites at 340 nm and emits at 488 nm, shifting to 360 nm and 460 nm when bound to double-stranded DNA; 6 month shelf life |
| Reaction Conditions / Protocol | Apply the solution to the sample, incubate briefly, wash, then examine under a fluorescence microscope or by flow cytometry |
| Storage Conditions | -20 °C, protected from light |
| Package Specifications | 10 mL | 50 mL |
| Key Features | Ready-to-use concentration; live and fixed cell compatible; 6 month shelf life |
For research use only, not for clinical use.
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