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| Product Name | DAPI Staining Solution, 1 mg/mL |
| Catalog No. | RERE-0239 |
| Description | Concentrated DAPI stock at 1 mg/mL for laboratories that prefer to set their own working dilution. Binding to double-stranded DNA produces a strong blue fluorescence, making the reagent suitable for nuclear counterstaining on fluorescence microscopy and for flow cytometric cell cycle work. Dilute the stock into buffer or medium to a working range of about 0.5 to 10 µg/mL before use. Store the concentrate at -20 °C away from light; it keeps for 12 months. |
| Intended Use | Preparation of working-strength DAPI nuclear stain for microscopy and flow cytometry |
| Principle / Technology | DAPI intercalates into double-stranded DNA and fluoresces blue upon ultraviolet excitation |
| Specificity | 1 mg/mL concentrated stock; free dye excites at 340 nm and emits at 488 nm, shifting to 360 nm and 460 nm when bound to double-stranded DNA; 12 month shelf life |
| Reaction Conditions / Protocol | Dilute the stock in buffer or medium to about 0.5 to 10 µg/mL, stain the sample, wash and image |
| Storage Conditions | -20 °C, protected from light |
| Package Specifications | 1 mL | 10 x 1 mL |
| Key Features | Concentrated stock; wide dilution latitude; 12 month shelf life |
For research use only, not for clinical use.
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