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| Product Name | DAPI Fluoromount-G Mounting Medium with Nuclear Counterstain |
| Catalog No. | ATRSA-0010 |
| Description | An aqueous-based antifade mounting medium containing DAPI (4',6-diamidino-2-phenylindole) nuclear counterstain for the simultaneous mounting and nuclear staining of immunofluorescence-labeled cells and tissue sections. |
| Intended Use | Mounting coverslips onto immunofluorescence-labeled slides while simultaneously staining cell nuclei blue for nuclear localization and cell counting in fluorescence microscopy. |
| Principle / Technology | DAPI binds to AT-rich regions of double-stranded DNA in the minor groove with approximately 20-fold fluorescence enhancement; excitation at 358 nm, emission at 461 nm; compatible with green (FITC), red (Texas Red), and far-red (Cy5) fluorophores. |
| Detection Method | Wide-field and confocal fluorescence microscopy with DAPI filter set (Ex 325–375 nm, Em 435–485 nm) |
| Sample Type | Fixed cells on coverslips, FFPE tissue sections on slides, cytospin preparations |
| Performance Range / Specifications | Bright nuclear staining with minimal cytoplasmic background at 0.5–2 µg/mL DAPI concentration |
| Sensitivity / LOD | N/A |
| Specificity | DNA specific; stains nuclei of all cell types; does not stain RNA-rich cytoplasm |
| Reaction Conditions / Protocol | Add one drop of DAPI Fluoromount-G to specimen on slide; carefully lower coverslip avoiding air bubbles; allow to cure at room temperature for 30 min or overnight at 4°C; seal edges with nail polish for long-term storage. |
| Components / Formulation | DAPI 0.5 µg/mL in Fluoromount-G aqueous mounting medium with anti-fading agents |
| Storage Conditions | 2–8°C, protect from light; warm to room temperature before use |
| Shelf Life | 12 months |
| Package Specifications | 20 mL dropper bottle |
| Product Form | Liquid mounting medium, ready to use |
| Quality Control | Fluorescence intensity and stability verification, spectral compatibility testing with common fluorophores, antifade performance |
| Key Features | Combined mounting and nuclear staining; antifade formulation; ready to use; compatible with multi-color immunofluorescence; water-based for convenience |
For research use only, not for clinical use.
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