- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Cytochrome C Apoptosis ELISA Detection Kit |
| Catalog No. | CCAT-HMM-0052 |
| Description | A sandwich enzyme immunoassay kit for the quantitative measurement of cytochrome c released from mitochondria into the cytoplasm during the early commitment phase of apoptosis. Cytochrome c translocation is a critical event that triggers apoptosome assembly and downstream caspase-9/caspase-3 cascade activation. |
| Intended Use | Quantification of mitochondrial cytochrome c release as an early apoptosis marker in cell fractionation studies, mechanism-of-action research for pro-apoptotic compounds, and mitochondrial dysfunction assessment. |
| Principle / Technology | During the intrinsic (mitochondrial) apoptosis pathway, pro-apoptotic Bcl-2 family proteins (Bax/Bak) form pores in the mitochondrial outer membrane, enabling cytochrome c release into the cytosol; the sandwich ELISA utilizes a capture antibody specific for cytochrome c on the microplate and a biotinylated detection antibody followed by streptavidin-HRP and TMB substrate for colorimetric quantification. |
| Detection Method | Colorimetric ELISA at 450 nm (TMB substrate; stop with acid) using a microplate spectrophotometer |
| Sample Type | Mitochondrial and cytosolic fractions from cultured mammalian cells; tissue homogenates from organs undergoing apoptosis; whole-cell lysates for total cytochrome c level measurement |
| Performance Range / Specifications | Quantification range: 0.16–10 ng/mL recombinant cytochrome c standard; cytosolic/mitochondrial fraction separation using differential centrifugation or commercial mitochondria isolation kits |
| Sensitivity / LOD | Sensitivity: approximately 0.1 ng/mL recombinant cytochrome c based on standard curve fitting |
| Specificity | Capture and detection antibodies demonstrate high specificity for mammalian cytochrome c; negligible cross-reactivity with cytochrome b, cytochrome oxidase, or other mitochondrial respiratory chain components; recombinant cytochrome c standard included for accurate inter-plate normalization |
| Reaction Conditions / Protocol | Harvest cells (2–5 × 10^6) and prepare cytosolic and mitochondrial fractions by differential centrifugation or mitochondria isolation kit; add 100 μL diluted fraction to antibody-coated ELISA well; incubate 2 hours at room temperature or overnight at 4°C; wash; add biotinylated detection antibody (1 hour); wash; add streptavidin-HRP (30 min); wash; add TMB substrate; stop with acid; read absorbance at 450 nm |
| Components / Formulation | Cytochrome C antibody pre-coated 96-well microplate (strip format), Biotinylated detection antibody concentrate, Streptavidin-HRP conjugate, Cytochrome C protein standard (lyophilized, recombinant), TMB substrate, Stop Solution, Wash Buffer (20× concentrate), Sample Diluent, 5× Cell Lysis Buffer |
| Storage Conditions | All kit unopened components at 2–8°C for 12 months; reconstituted cytochrome c standard at -80°C in single-use aliquots |
| Shelf Life | 12 months from date of manufacture |
| Package Specifications | 96-well plate (80 samples in duplicate with standard curve), 5 × 96-well plates |
| Product Form | Pre-coated microplate with concentrated liquid detection and enzyme conjugate components |
| Quality Control | Each lot standardized against recombinant cytochrome c reference material; inter-assay CV <12%; intra-assay CV <8%; plate-to-plate variation within 15% of mean for the standard curve |
| Key Features | Quantitative ELISA format enables apoptosis assessment without flow cytometry requirement; compatible with subcellular fractionation for mitochondrial vs. cytosolic localization; strip-well format allows partial plate use; significant dynamic range in samples from minimal to robust apoptosis induction |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
