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| Product Name | Cy5 NHS Ester |
| Catalog No. | RESK-0583 |
| Description | Cyanine 5 (Cy5) is a member of the cyanine fluorescent dye family and is a chemically synthesized polymethine dye. Cyanine dyes are currently supplied in two forms: non-sulfonated cyanines and sulfonated cyanines. The spectral properties of the two forms are almost identical, so they are interchangeable in most applications, such as labeling DNA, proteins and other biomolecules. The main difference is that sulfonated dyes are water-soluble, do not require an organic co-solvent in aqueous labeling reactions, and reduce the tendency of the dye to aggregate in water, thereby enhancing labeling efficiency. This Cyanine 5 (Cy5) is supplied in the sulfonated form. It is readily soluble in water, with Ex = 649 nm and Em = 670 nm, and offers bright fluorescence, strong photostability and pH insensitivity. It is suitable for labeling biomolecules such as peptides, proteins and nucleic acids for subsequent analysis of the target molecules by fluorescence imaging and other fluorescence-based biological methods. This Cy5 NHS ester is Cy5 bearing an N-hydroxysuccinimide (NHS) activated group, which reacts directly with amino groups (-NH2), mainly primary amines, on biomolecules to form stable amide bonds. |
| Intended Use | For covalent fluorescent labeling of peptides, proteins, nucleic acids and other amino-containing biomolecules for fluorescence imaging and other fluorescence-based analyses. |
| Principle / Technology | The N-hydroxysuccinimide (NHS) ester group of Cy5 NHS Ester reacts directly with primary amino groups (-NH2) on biomolecules to form stable amide bonds, covalently attaching the bright, photostable and pH-insensitive Cy5 fluorophore (Ex/Em 649/670 nm) to the target molecule. |
| Detection Method | Fluorescence imaging and other fluorescence-based detection methods (Ex/Em 649/670 nm). |
| Sample Type | Peptides, proteins, nucleic acids and other biomolecules bearing primary amino groups. |
| Performance Range / Specifications | Excitation wavelength (Ex): 649 nm Emission wavelength (Em): 670 nm Good water solubility (sulfonated form) High extinction coefficient Large molar absorptivity High specificity and sensitivity |
| Specificity | Reacts primarily with primary amino groups (-NH2) on biomolecules to form stable amide bonds. |
| Components / Formulation | Sulfonated Cy5 NHS ester |
| Storage Conditions | Store dry and protected from light at -25 to -15 °C. |
| Shelf Life | 1 year when stored dry and protected from light at -25 to -15 °C. |
| Package Specifications | 1 mg |
| Product Form | Solid |
| pH Range / Optimal pH | Fluorescence is pH-insensitive. |
| Expiration Date / Stability | Valid for 1 year when stored dry and protected from light at -25 to -15 °C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Sulfonated, water-soluble form; aqueous labeling reactions do not require an organic co-solvent. |
| Application Notes / Precautions | 1. Protect from light during handling and storage. 2. The sulfonated dye is water-soluble; no organic co-solvent is required for aqueous labeling reactions. 3. For your safety and health, wear a lab coat and disposable gloves during operation. |
For research use only, not for clinical use.
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