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| Product Name | Cy3 NHS Ester |
| Catalog No. | RESK-0567 |
| Description | Cyanine 3 (Cy3) is a member of the cyanine fluorescent dye family and a chemically synthesized polymethine dye. Cyanine dyes are currently supplied in two forms: non-sulfonated cyanines and sulfonated cyanines. The two forms have nearly identical spectral properties and are interchangeable for most applications, such as labeling DNA, proteins, and other biomolecules. The main difference is that sulfonated dyes are water-soluble, require no organic co-solvent in aqueous labeling reactions, are less prone to aggregation in water, and provide enhanced labeling efficiency. This Cyanine 3 (Cy3) product is supplied in the sulfonated form. It is readily soluble in water, with Ex = 555 nm and Em = 565 nm, and offers bright fluorescence, strong resistance to photobleaching, and pH insensitivity, making it suitable for labeling peptides, proteins, nucleic acids, and other biomolecules for subsequent fluorescence imaging and other fluorescence-based analyses of the target molecules. Cy3 NHS ester carries an N-hydroxysuccinimide (NHS) reactive group and reacts directly with amino groups (-NH2), primarily primary amines, on biomolecules to form stable amide bonds. |
| Intended Use | Fluorescent labeling of peptides, proteins, nucleic acids, and other amine-containing biomolecules for fluorescence imaging and other fluorescence-based biological analyses. |
| Principle / Technology | The N-hydroxysuccinimide (NHS) ester reactive group of Cy3 NHS ester reacts directly with amino groups (-NH2), primarily primary amines, on biomolecules to form stable amide bonds, covalently attaching the Cy3 fluorophore. |
| Detection Method | Fluorescence detection at Ex/Em 555/565 nm by fluorescence imaging and other fluorescence-based methods. |
| Sample Type | Peptides, proteins, nucleic acids, and other biomolecules containing primary amines. |
| Performance Range / Specifications | English Synonym: Cyanine 3, monosuccinimidyl ester Molecular Weight: 829.03 Appearance: purple solid Ex/Em: 555 nm/565 nm Solubility: soluble in DMSO, DMF, and water |
| Specificity | Reacts with amino groups (-NH2), primarily primary amines, forming stable amide bonds. |
| Reaction Conditions / Protocol | The sulfonated dye is water-soluble; aqueous labeling reactions do not require an organic co-solvent, which reduces dye aggregation and enhances labeling efficiency. The NHS ester reacts directly with primary amines on the target biomolecule to form stable amide bonds. |
| Components / Formulation | Cy3 NHS ester (Cyanine 3, monosuccinimidyl ester), sulfonated form. |
| Storage Conditions | Store at -20 °C, dry and protected from light. |
| Shelf Life | 1 year when stored as recommended. |
| Package Specifications | 1 mg |
| Product Form | Purple solid. |
| Molecular Weight | 829.03 |
| pH Range / Optimal pH | The dye fluorescence is pH-insensitive. |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Valid for 1 year when stored at -20 °C, dry and protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Spectrally interchangeable with non-sulfonated cyanine dyes for most applications; suitable for fluorescence imaging and other fluorescence-based biological analysis methods. |
| Application Notes / Precautions | Bright fluorescence, strong resistance to photobleaching, and pH insensitivity. Protect from light during storage and handling. |
For research use only, not for clinical use.
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