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| Product Name | Custom DNA Oligonucleotide Synthesis Service (50 nmol, HPLC Purified) |
| Catalog No. | PRIPRO-0002 |
| Description | A custom DNA oligonucleotide synthesis service at the 50 nanomole scale with high-performance liquid chromatography (HPLC) purification. HPLC purification removes truncated sequences, failure sequences, and depurination by-products, delivering oligos with superior purity compared to desalted grade. The process employs reversed-phase HPLC or ion-exchange HPLC depending on oligo length and modification status. Each oligo undergoes dual quality control by UV spectrophotometry and MALDI-TOF mass spectrometry. This grade is recommended for demanding applications such as qPCR primers, cloning, and in vitro transcription where sequence fidelity is critical. |
| Intended Use | Intended for quantitative real-time PCR (qPCR) primer pairs, molecular cloning with restriction enzyme sites, in vitro transcription template preparation, and any application requiring high-purity oligonucleotides. |
| Principle / Technology | Solid-phase phosphoramidite chemistry followed by reversed-phase or ion-exchange HPLC separation of full-length product from truncated failure sequences |
| Detection Method | HPLC chromatogram integration and MALDI-TOF MS |
| Sample Type | Template DNA or cDNA for downstream PCR amplification |
| Performance Range / Specifications | 2–80 bases; optimal purity for 10–60 nt range |
| Sensitivity / LOD | N/A — custom synthesis reagent |
| Specificity | Full-length oligo separated from n–1 and shorter failure sequences by HPLC; ≥95% full-length product |
| Reaction Conditions / Protocol | Dissolve in nuclease-free water or low-EDTA TE buffer. Prepare 100 µM stock solution. Use at 0.2–0.8 µM final concentration in qPCR. |
| Components / Formulation | One or two tubes containing lyophilized HPLC-purified oligonucleotide; COA included |
| Storage Conditions | –20 °C, protected from light and moisture |
| Shelf Life | 24 months dry at –20 °C; 6 months in solution at –20 °C |
| Package Specifications | Screw-cap tube with 50 nmol nominal scale yield (OD260 varies by sequence) |
| Product Form | Lyophilized white powder |
| Quality Control | HPLC purity ≥90% (full-length product); MALDI-TOF MS mass confirmation within ±0.05% of theoretical mass; A260/A280 ratio 1.8–2.0; endotoxin testing available upon request. |
| Key Features | HPLC purification ensures ≥90% full-length oligo; removal of n–1 and failure sequences; suitable for qPCR and high-fidelity applications; mass spectrometry confirmation included as standard; rush synthesis option available. |
| Purity | ≥90% by HPLC; ≥95% full-length by CE analysis |
| Concentration | Lyophilized from 50 nmol scale; typical yield 15–35 OD260 units |
| Activity / Unit Definition | Efficiently primes DNA synthesis by thermostable DNA polymerases; melting temperature (Tm) calculated by nearest-neighbor method |
| Molecular Weight | Calculated per sequence; included in certificate of analysis |
| Source / Origin | Fully synthetic; phosphoramidite building blocks from chemical synthesis |
| pH Range / Optimal pH | Resuspend in pH 7.0–8.5 buffer; avoid alkaline conditions >pH 9.0 |
| Shipping Conditions | Ambient temperature; HPLC-purified dry oligos stable during transit |
| Expiration Date / Stability | 24 months dry at –20 °C; 12 months at –20 °C in TE buffer |
| Regulatory / Compliance | ISO 9001 certified manufacturing facility; for research use only; not intended for human or animal diagnostic or therapeutic use |
| Compatibility | Universal compatibility with all commercial PCR and qPCR master mixes; tested with SYBR Green and probe-based detection chemistries |
| Recommended Buffer System | Low-EDTA TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0) or nuclease-free water |
| Application Notes / Precautions | Spin tube before opening. For qPCR, verify primer efficiency by standard curve method (90–110% efficiency recommended). Avoid contamination by using filter tips and dedicated work areas. |
| Batch-to-Batch Consistency | HPLC retention time reproducibility within ±2% across batches; mass accuracy maintained within ±0.05% |
For research use only, not for clinical use.
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