- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Crystal Violet Colony Staining Solution, 0.5% in 20% Ethanol |
| Catalog No. | ATRSA-0007 |
| Description | A ready-to-use crystal violet staining solution for the visualization and quantification of bacterial and mammalian cell colonies in colony formation assays, cell migration, and invasion experiments. |
| Intended Use | Staining and visualization of adherent cell colonies in clonogenic assays, soft agar colony formation assays, and cell migration/invasion Transwell assays for cancer biology and toxicology research. |
| Principle / Technology | Crystal violet (hexamethyl pararosaniline chloride) binds to negatively charged cell surface molecules and DNA, staining fixed cells deep purple; staining intensity correlates with cell number and can be quantified by dye extraction and spectrophotometry. |
| Detection Method | Visual colony counting, spectrophotometric quantification at 570 nm after dye solubilization |
| Sample Type | Fixed mammalian cell colonies in multi-well plates, soft agar colonies, Transwell membrane-attached cells |
| Performance Range / Specifications | Linear correlation between staining intensity and cell number 500–50,000 cells/well in 6-well format |
| Sensitivity / LOD | N/A |
| Specificity | N/A |
| Reaction Conditions / Protocol | Wash cells with PBS; fix with 4% paraformaldehyde or methanol for 10 min; add crystal violet solution for 10–20 min; rinse with water until background clear; air dry; for quantification, solubilize with 1% SDS or 10% acetic acid. |
| Components / Formulation | Crystal violet 0.5% w/v in 20% ethanol, 80% deionized water |
| Storage Conditions | 15–30°C, tightly sealed |
| Shelf Life | 24 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, ready to use |
| Quality Control | Absorbance spectrum verification, staining linearity with cell dilution series, lot-to-lot equivalence |
| Key Features | Ready to use; compatible with colony counting software; economical; validated for clonogenic and migration assays; no methanol needed |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
