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| Product Name | Crystal Violet Cell Staining Solution |
| Catalog No. | CATR-HMM-0044 |
| Description | A ready-to-use 0.5% Crystal Violet solution for the colorimetric quantification of adherent cell biomass. The triarylmethane protein-binding dye stains both viable and fixed cells, providing a measure of total cell density independent of metabolic state and well-suited for endpoint proliferation and cytotoxicity assays. |
| Intended Use | End-point quantification of total adherent cell mass in clonogenic survival assays, cell adhesion studies, and screening for antiproliferative compounds where metabolic-independent measurement of cell number is preferred. |
| Principle / Technology | Crystal Violet (hexamethyl pararosaniline chloride) binds to DNA and protein components of fixed or living cells through ionic and hydrophobic interactions; the bound dye is then extracted with a solubilization solution and measured colorimetrically at 590 nm. |
| Detection Method | Colorimetric absorbance measurement at 590 nm after dye elution using a microplate reader |
| Sample Type | Adherent mammalian cell lines; primary adherent cells in monolayer culture; colony formation assays; cell attachment assays in 6-well, 12-well, 24-well, or 96-well plates |
| Performance Range / Specifications | Linear range: OD 0.05–3.0 at 590 nm; proportional to cell density from subconfluent to confluent monolayers |
| Sensitivity / LOD | Detection of cell monolayers with surface coverage as low as 5% in 96-well format |
| Specificity | Crystal Violet stains all adherent material including fixed cells and extracellular matrix; signal is not dependent on metabolic activity, providing an orthogonal measurement to metabolism-based viability assays |
| Reaction Conditions / Protocol | Remove culture medium and wash cells gently with PBS; add 0.5% Crystal Violet solution to cover monolayer (50 μL for 96-well, 200 μL for 24-well); incubate 20 minutes at room temperature; wash extensively with water to remove unbound dye; air-dry plate; solubilize with 1% SDS or methanol; measure absorbance at 590 nm |
| Components / Formulation | 0.5% (w/v) Crystal Violet in 25% methanol and 75% deionized water, filtered to remove particulates; optional elution buffer (1% SDS in 50% ethanol) |
| Storage Conditions | Room temperature (15–25°C) protected from light; solution stable for 24 months; avoid freezing which may cause dye precipitation |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 100 mL, 500 mL, 1 L |
| Product Form | Ready-to-use aqueous-methanolic staining solution |
| Quality Control | Each lot tested for staining intensity ratio relative to reference cell line standards; pH verified (6.5–7.5); filtered through 0.22 μm membrane |
| Key Features | Simple and robust endpoint assay requiring only wash and elution steps; provides total biomass measurement independent of cellular metabolism; very low cost per sample; stained plates can be archived as permanent records; compatible with methanol or glutaraldehyde fixation before staining |
For research use only, not for clinical use.
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