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| Product Name | Coomassie Plus (Bradford) Protein Assay Kit |
| Catalog No. | PTR-HMM-0074 |
| Description | An improved Coomassie dye-based protein assay reagent formulated as a single, ready-to-use solution delivering rapid, linear colorimetric response for protein quantitation. The reagent is optimized for microplate and cuvette spectrophotometric measurement formats. |
| Intended Use | Rapid and accurate total protein concentration determination in cell lysates, tissue homogenates, column chromatography fractions, and purified protein preparations. |
| Principle / Technology | In an acidic environment, Coomassie G-250 dye binds primarily to arginine, histidine, lysine, tyrosine, tryptophan, and phenylalanine residues, shifting the dye's absorbance maximum from 465 nm (brown) to 595 nm (blue). |
| Detection Method | Absorbance measurement at 595 nm using microplate reader or spectrophotometer |
| Sample Type | Protein samples in common biological buffers; compatible with Tris, HEPES, PBS, and other common buffers; incompatible with high concentrations of detergents (>0.1% SDS or Triton X-100) |
| Performance Range / Specifications | Linear range: 100–1500 μg/mL (standard protocol); 1–25 μg/mL (micro protocol with proportionally less reagent) |
| Sensitivity / LOD | Lower limit of detection: approximately 100 μg/mL BSA equivalent in standard format |
| Specificity | Stains all proteins with varying intensity per unit mass depending on amino acid composition; BSA is recommended as the calibration standard for most purposes |
| Reaction Conditions / Protocol | Add 250 μL of reagent to 5 μL of sample in a microplate well or 1.5 mL of reagent to 30 μL of sample in a cuvette, mix gently, incubate at room temperature for 10 minutes, measure absorbance at 595 nm |
| Components / Formulation | Ready-to-use Coomassie dye reagent (950 mL), BSA standard (2 mg/mL, 5 x 1 mL vials), instruction manual |
| Storage Conditions | 2–8°C; the dye reagent should be brought to room temperature before use and gently mixed to dissolve any precipitated dye |
| Shelf Life | 18 months |
| Package Specifications | 950 mL (sufficient for 3,800 microplate assays or 630 cuvette assays) |
| Product Form | Ready-to-use dark brown liquid reagent |
| Quality Control | Each lot standardized against BSA; linearity of the standard curve verified (R² >0.99); tested for protein-to-protein variation using BSA and bovine gamma globulin standards |
| Key Features | Single reagent with no preparation required; room temperature incubation simplifies workflow; broad linear range accommodates diverse sample concentrations; compatible with standard laboratory equipment without specialized readers |
For research use only, not for clinical use.
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