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| Product Name | Color-Tracking SYBR qPCR Master Mix |
| Catalog No. | NATR-HMM-0192 |
| Description | A color-tracking SYBR qPCR system supplied with a blue master mix and yellow dilution buffer, producing a visible color change after template addition. |
| Intended Use | Relative gene-expression analysis; absolute quantification; dye-based real-time PCR requiring visual pipetting confirmation. |
| Principle / Technology | The blue qPCR master mix turns green when combined with template prepared in the yellow dilution buffer, while hot-start amplification and intercalating-dye detection support sensitive qPCR. |
| Detection Method | Intercalating-dye real-time PCR with visual setup tracking. |
| Sample Type | DNA or cDNA templates. |
| Performance Range / Specifications | 500 or 2,500 reactions at 20 µL per reaction. |
| Sensitivity / LOD | Supports single-digit-copy detection under suitable assay conditions. |
| Specificity | Hot-start DNA polymerase and an optimized specificity-enhancing buffer reduce nonspecific amplification. |
| Reaction Conditions / Protocol | Dilute the template with the supplied yellow buffer when applicable, add it to the blue master mix, confirm the expected green color, and run the compatible qPCR program. |
| Components / Formulation | Blue SYBR qPCR master mix, yellow template dilution buffer, hot-start DNA polymerase, intercalating dye and passive-reference system. |
| Storage Conditions | Store at −30 to −15°C protected from light. |
| Package Specifications | 500 reactions (20 µL per reaction); 2,500 reactions (20 µL per reaction). |
| Product Form | Color-tracking 2× SYBR qPCR master mix with dilution buffer. |
| Key Features | Visible template-addition confirmation; single-digit-copy sensitivity; hot-start specificity; two passive-reference options for instrument compatibility. |
| Expiration Date / Stability | After thawing, store the master mix at 2–8°C protected from light for up to 6 months. The mix remains stable through 30 freeze–thaw cycles. cDNA diluted in the supplied buffer remains stable for up to 7 days at −20°C, and a complete reaction mixture remains stable for up to 6 hours at 4°C. |
| Compatibility | Two passive-reference formulations support different real-time PCR instrument requirements. |
For research use only, not for clinical use.
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