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| Product Name | Co-Immunoprecipitation (Co-IP) Starter Kit with Magnetic Bead System |
| Catalog No. | PTR-HMM-0085 |
| Description | A comprehensive kit for performing co-immunoprecipitation experiments to study protein-protein interactions. The system includes antibody coupling magnetic beads, lysis buffer designed to preserve native protein complexes, and all necessary wash and elution reagents. The magnetic bead format streamlines separation steps and reduces non-specific background compared to traditional agarose-based methods. The kit is configured for use with user-supplied primary antibodies. |
| Intended Use | Isolation of native protein complexes from cell or tissue lysates for protein interaction analysis using antibody-coupled magnetic beads. |
| Principle / Technology | Affinity-based co-immunoprecipitation on antibody-conjugated magnetic beads |
| Detection Method | Western blot, mass spectrometry, or enzymatic activity assay of eluted complexes |
| Sample Type | Mammalian cell or tissue lysates prepared with non-denaturing lysis buffer |
| Performance Range / Specifications | Binding capacity: approximately 20–50 µg IgG per 50 µL bead slurry; typical protein yield: 1–5 µg per IP from 1 mg total lysate |
| Sensitivity / LOD | Detection of interaction partners at sub-stoichiometric levels in endogenous expression systems |
| Specificity | Specificity dependent on user-selected primary antibody; magnetic beads exhibit minimal non-specific protein binding |
| Reaction Conditions / Protocol | Antibody coupling: 30 min at room temperature; lysate incubation: 1 hr at 4 °C with gentle rotation; wash: 3 × 5 min; elution: 5 min at 95 °C with SDS loading buffer |
| Components / Formulation | Protein A/G magnetic bead slurry (2 mL), Co-IP lysis/wash buffer (100 mL), neutral elution buffer, SDS-PAGE elution buffer, protease inhibitor cocktail (100×), microcentrifuge tube magnetic rack |
| Storage Conditions | Beads and inhibitors: 2–8 °C; buffers: room temperature or 2–8 °C per label |
| Shelf Life | 12 months |
| Package Specifications | 40 reactions kit (50 µL bead slurry per reaction) |
| Product Form | Liquid reagents and magnetic bead suspension |
| Quality Control | Each lot validated using an anti-c-Myc antibody IP from transfected HEK293 lysate; co-immunoprecipitated partner detected by Western blot |
| Key Features | Magnetic bead system enables gentle washing that preserves weak and transient interactions |
| Purity | As specified per product type; recombinant proteins ≥90% by SDS-PAGE |
| Concentration | As specified on product label or certificate of analysis |
| Activity / Unit Definition | Binding capacity or enzymatic activity as specified per product documentation |
| Molecular Weight | Varies by product as specified in documentation |
| Source / Origin | Recombinant expression in E. coli, mammalian, or insect cell systems; synthetic chemistry for small molecule reagents |
| pH Range / Optimal pH | pH 7.0–8.0 for most protein applications |
| Shipping Conditions | Ambient for dry reagents; cold packs for liquid protein solutions; dry ice for frozen products |
| Expiration Date / Stability | 12–24 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 quality system |
| Compatibility | Compatible with standard SDS-PAGE, Western blot, mass spectrometry, and chromatography systems |
| Recommended Buffer System | PBS, Tris, or HEPES buffers as specified in product protocol |
| Application Notes / Precautions | Avoid repeated freeze-thaw cycles for protein solutions; use fresh aliquots; protect light-sensitive reagents |
| Batch-to-Batch Consistency | Protein concentration and activity within ±15% of reference lot |
For research use only, not for clinical use.
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