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| Product Name | CM-DiI Live Cell Tracking Dye |
| Catalog No. | RESK-0582 |
| Description | CM-DiI is a lipophilic carbocyanine dye commonly used to label cell membranes in a variety of biological and neuroscience research applications. It is a fluorescent dye that exhibits strong fluorescence when incorporated into cell membranes or lipid-containing structures. CM-DiI is widely used to label and track cell membranes for the study of cell migration, cell tracking and axonal projections in living and fixed tissues. Once incorporated into the cell membrane, CM-DiI tends to stay in place and does not diffuse rapidly within the membrane, a property that enables long-term imaging and tracking studies. CM-DiI emits intense red fluorescence, making it easy to detect and suitable for fluorescence microscopy. CM-DiI is relatively stable, and its fluorescence is resistant to photobleaching, allowing extended imaging periods without significant signal loss. It enables researchers to visualize and analyze labeled structures with high specificity. CM-DiI can be used in cell cultures and in living organisms, allowing researchers to study biological processes of varying complexity. |
| Intended Use | For labeling and tracking of cell membranes in live and fixed samples, including studies of cell migration, cell tracking and axonal projections in cell cultures and living organisms. |
| Principle / Technology | CM-DiI is a lipophilic carbocyanine dye that inserts into the lipid bilayer of cell membranes via its lipid tail. Upon incorporation it exhibits strong red fluorescence and remains largely in place without rapid lateral diffusion within the membrane, enabling long-term imaging and tracking. The dye is compatible with aldehyde fixation, allowing labeled cells to be analyzed in fixed tissues. |
| Detection Method | Fluorescence microscopy (red fluorescence, Ex/Em 553/570 nm). |
| Sample Type | Cultured cells, living organisms and fixed tissues. |
| Performance Range / Specifications | Appearance: orange-red solid Molecular weight: 1051.5 Solvent: DMSO Ex/Em: 553/570 nm |
| Components / Formulation | CM-DiI dye (supplied as an orange-red solid); dissolve in DMSO before use. |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 1 year when stored at -25 to -15 °C. |
| Package Specifications | 1 x 50 μg; 5 x 50 μg |
| Product Form | Solid (orange-red) |
| Molecular Weight | 1051.5 |
| Expiration Date / Stability | Valid for 1 year when stored at -25 to -15 °C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Compatible with live-cell labeling and subsequent aldehyde fixation; suitable for fluorescence microscopy. |
| Application Notes / Precautions | 1. This product is not sterile. For live-cell labeling and subsequent co-culture, filter the dye solution before use; a 0.22 μm filter is recommended. 2. Dissolve the dye in DMSO before use. 3. Protect from light during handling and storage to preserve fluorescence. |
For research use only, not for clinical use.
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