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| Product Name | CFSE Cell Proliferation Tracking Dye Kit |
| Catalog No. | CATR-HMM-0050 |
| Description | A fluorescent cell labeling kit using carboxyfluorescein succinimidyl ester for long-term tracking of cell division by flow cytometry. CFSE passively diffuses into cells where intracellular esterases cleave the acetate groups, converting it to a membrane-impermeant fluorescent form that covalently binds to intracellular amines. Each cell division halves the fluorescence intensity of daughter cells, enabling determination of proliferation history through fluorescence peak analysis. |
| Intended Use | Longitudinal tracking of lymphocyte and other cell proliferation through progressive fluorescence dilution upon cell division. |
| Principle / Technology | Covalent intracellular fluorescent labeling with fluorescence halving at each cell division |
| Detection Method | Flow cytometry (FITC channel, 488 nm excitation) |
| Sample Type | Peripheral blood mononuclear cells, splenocytes, lymph node cells, and cultured cell lines |
| Performance Range / Specifications | Fluorescence labeling detectable through 8–10 cell divisions; labeling efficiency ≥99% of viable cells |
| Sensitivity / LOD | Detection of individual cell divisions through distinct fluorescence peaks in flow cytometry histograms |
| Specificity | Labels all viable cells non-selectively; intracellular retention permits multi-day tracking without significant dye leakage |
| Reaction Conditions / Protocol | Suspend cells at 1 × 10⁶ cells/mL in PBS/0.1% BSA; add CFSE to 5 µM final; incubate 10 min at 37 °C; quench with 5 volumes cold complete medium; wash 2× before culture |
| Components / Formulation | CFSE dye (lyophilized, 50 µg/vial), anhydrous DMSO for reconstitution |
| Storage Conditions | Lyophilized dye: –20 °C desiccated, protected from light; reconstituted stock: –20 °C single-use aliquots |
| Shelf Life | 12 months lyophilized; 1 month reconstituted at –20 °C |
| Package Specifications | 5 × 50 µg vials |
| Product Form | Lyophilized dye |
| Quality Control | Each lot tested on human PBMCs; labeling uniformity (CV of fluorescence intensity) ≤10% across labeled population |
| Key Features | Covalent protein binding ensures fluorescence retention without intercellular dye transfer |
| Purity | Reagent grade; dye purity ≥95% by HPLC; sterile where applicable |
| Concentration | As specified on product label; working concentrations optimized per protocol |
| Activity / Unit Definition | Signal-to-noise ratio and linearity verified on reference cell lines per lot |
| Molecular Weight | Varies by dye or reagent component as specified |
| Source / Origin | Synthetic dyes and tetrazolium compounds; recombinant enzymes where applicable |
| pH Range / Optimal pH | pH 7.2–7.4 for cell-based assays |
| Shipping Conditions | Cold packs; protect light-sensitive reagents from light |
| Expiration Date / Stability | 6–12 months at recommended storage temperature |
| Regulatory / Compliance | Research use; manufactured under ISO 9001 |
| Compatibility | Compatible with adherent and suspension mammalian cell culture in 96-well and 384-well formats |
| Recommended Buffer System | PBS or phenol red-free complete medium as specified in protocol |
| Application Notes / Precautions | Optimize cell seeding density; include appropriate vehicle controls; avoid phenol red interference with absorbance-based assays |
| Batch-to-Batch Consistency | Signal linearity R² ≥0.99 with reference cell number standard curve per lot |
For research use only, not for clinical use.
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