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Cat.No: DB-0549 Datasheet Instruction for Use
Specification Quantities
Price $520.59–867.65
| Product Name | Cell Free DNA Extraction Kit with Magnetic Beads |
| Catalog No. | DB-0549 |
| Description | The cell-free DNA isolation kit with magnetic beads is a kit that uses magnetic beads coated with a novel nucleic acid purification medium to stably, efficiently, and conveniently isolate cell-free DNA (cfDNA) from serum and plasma samples. |
| Kit Components | Magnetic Beads: 1 ml Lysis Buffer: 10 ml Wash Buffer I (add 7ml anhydrous ethanol before first use): 21 ml (+7 ml) Wash Buffer II (add 24ml anhydrous ethanol before first use): 16 ml (+24 ml) Elution Buffer: 5 ml Proteinase K: 0.5 ml Manual: 1 copy |
| Test Procedure | Take 20–200 µl of serum or plasma sample, add 200 µl of lysis buffer and 10 µl of proteinase K, mix by vortexing or shaking, and incubate at 56°C for 10 minutes to lyse the cells. Cool to room temperature. Add 200 µl of anhydrous ethanol to each tube, followed by 20 µl of magnetic bead suspension (be sure to mix well before use). Gently mix, then let stand at room temperature for 5 minutes, gently inverting the tube 1–2 times during this period. Place the centrifuge tube in the magnetic field of a magnetic stand. Once the magnetic beads have completely aggregated, carefully aspirate the supernatant. White flocculent particles may appear after adding ethanol; this is normal. Be sure to vortex or shake the magnetic beads thoroughly before use. If necessary, increase the amount of magnetic beads or extend the binding time to improve the yield. Add 500 µl of Wash Solution I, gently shake to disperse the magnetic beads, invert the tube twice, then place the centrifuge tube in the magnetic field of the magnetic stand. Once the magnetic beads have completely aggregated, aspirate as much of the supernatant as possible. If magnetic beads are adhering to the inside of the tube cap, hold the entire tube and invert it twice to ensure the beads are fully adsorbed, then discard the supernatant. Add 600 µl of Wash Solution II, gently shake to disperse the magnetic beads, invert the tube twice, then place it in the magnetic field of the magnetic rack. Once the magnetic beads have completely aggregated, aspirate as much of the supernatant as possible. Allow the centrifuge tube to stand at room temperature for 5–10 minutes, or place it in a 37°C forced-air oven for 5 minutes, to ensure that any residual ethanol or other trace liquids have completely evaporated. Add 35–50 µl of elution buffer, gently vortex to suspend the magnetic beads in the solution, and incubate at room temperature for 3–5 minutes, gently shaking the tube 1–2 times during this period. Place the centrifuge tube in a magnetic field. Once the magnetic beads have completely aggregated, carefully transfer the solution to a new centrifuge tube and store it at -20°C. The resulting solution is high-purity cell-free DNA. To obtain a higher concentration of the sample, the volume of elution buffer can be reduced to 20 μL; however, the amount of eluted DNA will be correspondingly reduced. When room temperature is low, preheating the elution buffer briefly at 37°C can improve the yield. Additionally, returning the eluted solution to the original magnetic beads and performing a second elution can increase the yield by approximately 10–30%; alternatively, using fresh elution buffer for a second elution after the first elution will yield approximately 15–40% of the DNA obtained in the first elution. |
| Storage and Transportation | Protease K: Store at -20°C. All other items should be stored at room temperature and are valid for one year. Magnetic beads that will not be used for an extended period may be stored at 4°C, where they will remain stable for a longer period. |
For research use only, not for clinical use.
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