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| Product Name | CCK-8 Cell Counting Kit (WST-8) |
| Catalog No. | CATR-HMM-0036 |
| Description | A highly sensitive, single-solution cell viability assay using the water-soluble tetrazolium salt WST-8. The WST-8 reagent produces a soluble orange formazan dye upon reduction by cellular dehydrogenases, eliminating the need for organic solvent dissolution steps required by MTT. |
| Intended Use | High-throughput cell proliferation and cytotoxicity screening in drug discovery, toxicology studies, and cell biology research using mammalian cell cultures. |
| Principle / Technology | WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium) is reduced by NADH/NADPH-dependent dehydrogenases in viable cells to a water-soluble formazan dye with absorbance at 450 nm; an intermediate electron carrier (1-methoxy PMS) enhances the reaction rate. |
| Detection Method | Colorimetric absorbance measurement at 450 nm using a microplate reader with reference wavelength at 600–650 nm |
| Sample Type | Adherent and suspension cells, primary cells, co-culture systems, 3D spheroid cultures grown in 96-well or 384-well plates |
| Performance Range / Specifications | Linear detection: 100–100,000 cells per well; stable color development over 1–4 hours |
| Sensitivity / LOD | Approximately 100–200 viable cells per well for most adherent cell lines |
| Specificity | Reduction activity is proportional to the number of metabolically competent cells; minimal interference from phenol red when read at 450 nm against reference wavelength |
| Reaction Conditions / Protocol | Add 10 μL CCK-8 solution per 100 μL culture medium directly to cells; incubate at 37°C for 1–4 hours depending on cell type and density; measure absorbance at 450 nm; no washing or medium removal required |
| Components / Formulation | Ready-to-use WST-8 reagent containing WST-8 and 1-methoxy-5-methylphenazinium methylsulfate (1-methoxy PMS) in PBS, supplied in sterile amber bottle |
| Storage Conditions | 2–8°C protected from light; avoid repeated freeze-thaw; warm to room temperature before use; do not expose to direct sunlight |
| Shelf Life | 12 months from date of manufacture |
| Package Specifications | 1 mL, 5 mL, 10 mL, 100 mL (100 tests, 500 tests, 1,000 tests, or 10,000 tests in 96-well format) |
| Product Form | Sterile ready-to-use liquid solution in amber vial |
| Quality Control | Each lot is validated using HeLa and Jurkat cell lines; linearity confirmed across four-log dynamic range; sterility tested per USP guidelines |
| Key Features | Single-step homogeneous assay requiring no washing, lysis, or organic solvents; non-toxic to cells allowing further downstream analysis; higher sensitivity than MTT, MTS, or XTT; compatible with multiplexed assays |
For research use only, not for clinical use.
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