Bright Red NHS Ester Fluorescent Labeling Dye (Ex/Em 590/617 nm)
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Bright Red NHS Ester Fluorescent Labeling Dye (Ex/Em 590/617 nm)

Cat.No: RERE-0007 Datasheet

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Product Name Bright Red NHS Ester Fluorescent Labeling Dye (Ex/Em 590/617 nm)
Catalog No. RERE-0007
Description A bright red fluorophore supplied as the amine-reactive NHS ester. It labels the primary amine groups of proteins, amine-modified oligonucleotides and other amine-bearing molecules, and is most often applied to protein and antibody tagging. Emission at 617 nm keeps the signal clear of green channels, so it pairs well with green- and orange-emitting labels in multi-colour experiments.
Intended Use Primary-amine labelling of proteins, antibodies, amine-modified oligonucleotides and other amine-containing molecules.
Principle / Technology The NHS ester forms stable amide bonds with primary amines (R-NH2), attaching the bright red fluorophore to the target molecule.
Detection Method Fluorescence detection with excitation/emission of 590/617 nm.
Sample Type Purple solid.
Performance Range / Specifications CAS 1638544-48-5; molecular formula C39H35N3O13S2(2-); molecular weight 817.84 g/mol; appearance purple solid; purity ≥70%; Ex/Em 590/617 nm.
Specificity Selective for primary amines; red emission with good separation from green and orange labels.
Reaction Conditions / Protocol Prepare a dry stock in anhydrous solvent, react with the amine-bearing substrate under the chosen buffer conditions, then purify the conjugate away from free dye.
Components / Formulation One vial of dye; solvents and purification media are provided by the user.
Storage Conditions Store at -25 to -15 °C, protected from light.
Shelf Life 12 months under the recommended storage conditions.
Package Specifications 1 mg, 5 mg, 10 mg or 50 mg
Product Form Purple solid.
Quality Control Released at purity ≥70% against the documented specification.
Key Features - Bright red emission clear of green and orange channels
- Direct primary-amine coupling
- Four pack sizes from 1 mg to 50 mg
- Suits protein and antibody tagging
- One-year shelf life protected from light
Purity ≥70%
Activity / Unit Definition No unit definition; performance is defined by its spectra and amine coupling.
Molecular Weight CAS 1638544-48-5; molecular formula C39H35N3O13S2(2-); molecular weight 817.84 g/mol.
Source / Origin Synthetic small-molecule fluorophore, supplied as a purified solid.
pH Range / Optimal pH Does not apply; pH is not a meaningful specification for this product type.
Expiration Date / Stability Stable through the stated shelf life when kept protected from light at the recommended storage temperature.
Regulatory / Compliance Restricted to research applications.
Compatibility Compatible with standard fluorescence instrumentation - microscopes, plate readers, flow cytometers or imaging systems as appropriate to the format.
Recommended Buffer System No buffer is included; prepare anhydrous stock fresh before use.
Application Notes / Precautions Handle protected from light, minimise freeze-thaw cycles by preparing aliquots, and verify that the detection channel matches the dye before use.
Batch-to-Batch Consistency Each production lot is released against fixed acceptance criteria, keeping performance consistent between orders.
Model Type Amine-reactive fluorescent dye (NHS ester)
Starting Material / Tissue Site Synthesised red fluorophore, esterified as the NHS ester
Delivery Standard Pack sizes 1 mg, 5 mg, 10 mg and 50 mg
Turnaround Time No turnaround figure is published; shipping is arranged when the order is confirmed.
Readouts / Downstream Assays Excitation/emission 590/617 nm on a red channel.

For research use only, not for clinical use.

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