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| Product Name | BCIP p-Toluidine Salt |
| Catalog No. | RERE-0046 |
| Description | 5-Bromo-4-chloro-3-indolyl phosphate, supplied as the p-toluidine salt, is the standard chromogenic substrate of alkaline phosphatase. It is commonly paired with an oxidant such as INT or nitrotetrazolium blue to boost detection sensitivity. Under enzyme catalysis BCIP is first dephosphorylated to a reactive intermediate that oxidises and dimerises into an indigo dye, and the reducing equivalents released in that dimerisation reduce the tetrazolium salt to an insoluble purple formazan, giving equipment free visual detection. The powder dissolves in 100% DMF rather than water. |
| Intended Use | Alkaline phosphatase based chromogenic detection on Western, Northern and Southern blots, in situ hybridization, immunohistochemistry and identification of induced pluripotent stem cell colonies. |
| Principle / Technology | Alkaline phosphatase hydrolyses BCIP to a reactive intermediate that dimerises into an indigo dye; the paired tetrazolium salt captures the released reducing equivalents and precipitates as a deep purple formazan. |
| Detection Method | Alkaline phosphatase labelled probes and antibodies |
| Sample Type | Blot membranes, tissue sections, cell preparations and stem cell colonies |
| Performance Range / Specifications | Equipment free readout with deep purple to blue purple deposition when paired with NBT or INT. |
| Specificity | Colour development requires alkaline phosphatase activity at the probe binding site. |
| Reaction Conditions / Protocol | Dissolve the powder in 100% DMF to prepare the substrate solution, combine with the tetrazolium salt in the designated buffer and incubate the specimen until colour develops. |
| Storage Conditions | Powder at -20 °C, dry and protected from light for 3 years; stock solution at 20 mg/mL holds at 4 °C protected from light for 6 months |
| Shelf Life | 3 years (powder) |
| Package Specifications | 100 mg |
| Product Form | White to pale yellow powder |
| Quality Control | Purity certified at 98% or higher. |
| Key Features | High purity p-toluidine salt; pairs with NBT or INT for amplified signal; broad blot and tissue compatibility. |
| Purity | >=98% |
| Molecular Weight | 433.62 Da |
| Regulatory / Compliance | For research use. |
| Compatibility | Insoluble in water; soluble in 100% DMF. |
| Batch-to-Batch Consistency | Lot specific certificate of analysis. |
For research use only, not for clinical use.
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