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| Product Name | BCA Protein Quantification Assay Kit |
| Catalog No. | PTR-HMM-0055 |
| Description | A bicinchoninic acid (BCA)-based colorimetric assay kit for the quantification of total protein concentration in biological samples. This reagent system provides accurate and reproducible measurements across a broad range of protein concentrations with minimal interference from common buffer components. |
| Intended Use | Total protein quantification in cell lysates, tissue homogenates, serum, plasma, and purified protein preparations for biochemical and molecular biology applications. |
| Principle / Technology | Reduction of Cu2+ to Cu+ by protein in alkaline medium, followed by colorimetric detection of Cu+ using bicinchoninic acid, producing a purple-colored complex with absorbance at 562 nm. |
| Detection Method | Colorimetric measurement at 562 nm using standard spectrophotometer or microplate reader |
| Sample Type | Cell lysates, tissue extracts, serum, plasma, purified proteins, culture supernatants |
| Performance Range / Specifications | Linear detection range: 20–2000 μg/mL (standard protocol); 0.5–20 μg/mL (enhanced protocol with 60°C incubation) |
| Sensitivity / LOD | ≤5 μg/mL (standard protocol) |
| Specificity | Universal protein detection with minimal protein-to-protein variation; compatible with most detergents (≤1% SDS, Triton X-100, Tween-20) |
| Reaction Conditions / Protocol | Mix sample with working reagent, incubate at 37°C for 30 minutes (or 60°C for 30 minutes for enhanced sensitivity), cool to room temperature, read absorbance |
| Components / Formulation | BCA Reagent A (sodium carbonate, sodium bicarbonate, bicinchoninic acid, sodium tartrate in 0.1 M NaOH), BCA Reagent B (4% cupric sulfate solution), bovine serum albumin (BSA) standard set (2 mg/mL stock with dilutions), 96-well microplate or cuvette protocol guide |
| Storage Conditions | 2–8°C; BCA working reagent should be prepared fresh before each use; BSA standard stable at 2–8°C for 12 months after opening |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 500 tests (microplate format) or 250 tests (cuvette format) |
| Product Form | Liquid reagents; standard provided as lyophilized BSA |
| Quality Control | Each lot is tested against NIST-traceable protein standards; linearity verified across the full assay range; endotoxin tested (<0.1 EU/mg BSA) |
| Key Features | Broad compatibility with reducing agents and detergents (≤5 mM DTT, ≤1% SDS); color stable for up to 1 hour after development; no heating required for standard protocol |
For research use only, not for clinical use.
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