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| Product Name | BCA Protein Assay Standard Set (BSA Pre-Diluted) |
| Catalog No. | PTR-HMM-0066 |
| Description | A set of pre-diluted bovine serum albumin (BSA) standards calibrated for use with the BCA protein assay. These ready-to-use standards eliminate the need for serial dilution preparation and reduce pipetting errors. |
| Intended Use | Generation of a standard curve for accurate protein concentration determination using the BCA protein assay method. |
| Principle / Technology | Precisely formulated BSA solutions at known concentrations, calibrated against NIST-traceable reference materials, designed to produce a linear standard curve for BCA assay protein quantification. |
| Detection Method | Colorimetric detection at 562 nm with BCA working reagent; color intensity proportional to protein concentration |
| Sample Type | These are standard solutions for calibration purposes, not biological samples |
| Performance Range / Specifications | Concentration range: 125–2000 μg/mL (7 pre-diluted standards plus blank); standard curve R² ≥0.99 when used correctly |
| Sensitivity / LOD | Lower limit of reliable quantification: 125 μg/mL BSA equivalent |
| Specificity | BSA-based standard; for more accurate quantification of specific target proteins, a matched protein standard is recommended |
| Reaction Conditions / Protocol | Pipette 25 μL of each standard into microplate wells, add 200 μL BCA working reagent, incubate at 37°C for 30 minutes, read at 562 nm, generate standard curve |
| Components / Formulation | Seven pre-diluted BSA standards (2000, 1500, 1000, 750, 500, 250, 125 μg/mL) and one blank (0 μg/mL) in protein-free buffer with stabilizers, provided in ready-to-use vials |
| Storage Conditions | 2–8°C; standards are stable for 6 months after opening when stored at 2–8°C |
| Shelf Life | 18 months from date of manufacture |
| Package Specifications | Set of 8 vials (1 mL each), sufficient for 320 standard curve points |
| Product Form | Ready-to-use liquid standards in color-coded vials |
| Quality Control | Each lot calibrated against NIST Standard Reference Material 927e (BSA); accuracy verified within ±5% of assigned values; endotoxin tested |
| Key Features | Pre-diluted standards eliminate pipetting and dilution errors; color-coded vials aid quick identification; extended opened-vial stability reduces waste in low-throughput laboratories |
For research use only, not for clinical use.
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