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| Product Name | ATTO 425 NHS Ester (Coumarin Fluorescent Dye) |
| Catalog No. | RERE-0009 |
| Description | This is the amine-reactive ester of the ATTO 425 coumarin dye. It carries the same high photostability and moderate hydrophilicity as the parent dye and couples directly to primary amine groups, so proteins, amine-modified oligonucleotides and other amine-bearing molecules can be tagged without additional activation steps. Blue emission at 485 nm places it in the standard blue channel of most imaging systems. |
| Intended Use | Primary-amine labelling of proteins, amine-modified oligonucleotides and other amine-containing molecules. |
| Principle / Technology | The NHS ester reacts with primary amines to form stable amide bonds, carrying the photostable coumarin fluorophore onto the target molecule. |
| Detection Method | Fluorescence detection with excitation/emission of 439/485 nm. |
| Sample Type | Yellow solid. |
| Performance Range / Specifications | CAS 892156-28-4; molecular formula C26H30N2O8; molecular weight 498.53 g/mol; appearance yellow solid; purity ≥95%; Ex/Em 439/485 nm; high photostability; moderate hydrophilicity. |
| Specificity | Selective for primary amines; blue coumarin emission suited to multi-colour panels. |
| Reaction Conditions / Protocol | Dissolve in anhydrous DMSO shortly before use, react with the amine-bearing substrate, then separate the labelled product from free dye. |
| Components / Formulation | One vial of dye; solvents and purification media are provided by the user. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 1 mg, 5 mg or 10 mg |
| Product Form | Yellow solid. |
| Quality Control | Released at purity ≥95%. |
| Key Features | - Coumarin photostability with direct amine coupling - Blue 485 nm emission fits standard instrument channels - Purity released at ≥95% - Moderate hydrophilicity eases handling in buffer - Three pack sizes from 1 mg to 10 mg |
| Purity | ≥95% |
| Activity / Unit Definition | No unit definition; characterised by photostability and spectral behaviour. |
| Molecular Weight | CAS 892156-28-4; molecular formula C26H30N2O8; molecular weight 498.53 g/mol. |
| Source / Origin | Synthetic small-molecule fluorophore, supplied as a purified solid. |
| Expiration Date / Stability | Stability is maintained by the recommended storage temperature together with continuous protection from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Read-outs run on routine fluorescence equipment without proprietary hardware. |
| Recommended Buffer System | No buffer is included; prepare anhydrous stock fresh before use. |
| Application Notes / Precautions | Protect from light at all times, both in storage and during labelling, and avoid repeated warming cycles. Prepare stock solutions fresh where possible and confirm the instrument filter set before starting an experiment. |
| Batch-to-Batch Consistency | Lot-to-lot consistency is controlled through defined manufacturing specifications; each lot ships with its release record. |
| Model Type | Amine-reactive fluorescent dye (NHS ester) |
| Starting Material / Tissue Site | Synthesised coumarin fluorophore, esterified as the NHS ester |
| Delivery Standard | Pack sizes 1 mg, 5 mg and 10 mg |
| Turnaround Time | No turnaround figure is published; shipping is arranged when the order is confirmed. |
| Readouts / Downstream Assays | Excitation/emission 439/485 nm on a blue channel. |
For research use only, not for clinical use.
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