- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | ATTO 425 Carboxylic Acid (Coumarin Fluorescent Dye) |
| Catalog No. | RERE-0008 |
| Description | ATTO 425 belongs to a new generation of coumarin dyes and stands out for high photostability together with moderate hydrophilicity. The acid form supplies a free carboxyl group as a versatile handle for coupling chemistry, letting the laboratory attach the blue-emitting fluorophore through the chemistry of its choice. Applications listed on the page include fluorescence microscopy. |
| Intended Use | Carboxyl-handle blue fluorophore for coupling chemistry and fluorescence microscopy applications. |
| Principle / Technology | The free carboxylic acid acts as the coupling handle; after activation it forms amide linkages with primary amines, and the coumarin core provides a photostable blue signal. |
| Detection Method | Fluorescence detection with excitation/emission of 439/485 nm. |
| Sample Type | Yellow solid. |
| Performance Range / Specifications | CAS 652966-03-5; molecular formula C22H27NO6; molecular weight 401.18 g/mol; appearance yellow solid; purity ≥95%; Ex/Em 439/485 nm; high photostability; moderate hydrophilicity. |
| Specificity | Blue coumarin emission with strong photostability; carboxyl chemistry rather than amine-reactive ester form. |
| Reaction Conditions / Protocol | Activate the carboxyl group under the coupling chemistry of choice, react with the amine-bearing substrate, then purify the conjugate. |
| Components / Formulation | One vial of dye; activation reagents and buffers are not included. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 1 mg, 5 mg or 10 mg |
| Product Form | Yellow solid. |
| Quality Control | Released at purity ≥95%. |
| Key Features | - New-generation coumarin with high photostability - Moderate hydrophilicity eases handling in buffer - Purity released at ≥95% - Free acid handle for custom coupling chemistry - Three pack sizes from 1 mg to 10 mg |
| Purity | ≥95% |
| Activity / Unit Definition | No unit definition; characterised by photostability and spectral behaviour. |
| Molecular Weight | CAS 652966-03-5; molecular formula C22H27NO6; molecular weight 401.18 g/mol. |
| Source / Origin | Synthetic small-molecule fluorophore, supplied as a purified solid. |
| Expiration Date / Stability | Remains stable for the stated period provided the cold chain is not broken and light exposure is avoided. |
| Regulatory / Compliance | Supplied for laboratory research purposes. |
| Compatibility | Works on common fluorescence platforms; match the filter set to the excitation/emission values listed for this product. |
| Recommended Buffer System | No buffer is included; activate and couple under the user's chosen chemistry. |
| Application Notes / Precautions | Wear gloves when handling DMSO stocks, keep vials in the dark, and let chilled vials warm to room temperature before opening to avoid condensation. |
| Batch-to-Batch Consistency | Consistency between lots is maintained by fixed production controls and release testing against specification. |
| Model Type | Carboxyl-handle fluorescent dye (acid form) |
| Starting Material / Tissue Site | Synthesised coumarin fluorophore in the free acid form |
| Delivery Standard | Pack sizes 1 mg, 5 mg and 10 mg |
| Turnaround Time | dispatch timing is settled at purchase. |
| Readouts / Downstream Assays | Excitation/emission 439/485 nm on a blue channel. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
