- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Antigen Coating Buffer, Carbonate-Based, 0.05 M, pH 9.6 |
| Catalog No. | ATRD-0002 |
| Description | An alkaline carbonate-bicarbonate binding buffer optimized for the passive adsorption of proteins and peptides to polystyrene ELISA plate surfaces through ionic and hydrophobic interactions. |
| Intended Use | Coating capture antibodies or protein antigens onto 96-well and 384-well microtiter plates for indirect and sandwich ELISA formats. |
| Principle / Technology | Alkaline pH above the pI of most proteins promotes a net negative charge on protein molecules, enhancing ionic binding to positively charged regions on treated polystyrene surfaces. |
| Detection Method | ELISA signal-to-noise ratio, coating uniformity by well-to-well CV measurement |
| Sample Type | Purified monoclonal and polyclonal antibodies, recombinant proteins, synthetic peptides |
| Performance Range / Specifications | Coating efficiency >90% for IgG at 1–10 µg/mL; well-to-well CV <5% |
| Sensitivity / LOD | N/A |
| Specificity | Optimized for high-binding polystyrene plates; verify compatibility with other plate types |
| Reaction Conditions / Protocol | Add 100 µL per well; incubate overnight at 4°C or 2 hours at 37°C in humidified chamber; wash 3x with PBST before blocking. |
| Components / Formulation | Sodium carbonate 15 mM, sodium bicarbonate 35 mM in ultrapure water, pH 9.6, 0.02% sodium azide |
| Storage Conditions | 2–8°C |
| Shelf Life | 12 months |
| Package Specifications | 100 mL, 500 mL |
| Product Form | Liquid, ready to use |
| Quality Control | pH ±0.05, coating validation with reference antibody panel, preservative concentration verification |
| Key Features | Standard ELISA coating buffer; ready to use; validated with multiple antibody isotypes; compatible with overnight and rapid coating protocols |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
