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| Product Name | Ammonium Sulfate Protein Precipitation Reagent |
| Catalog No. | PTR-HMM-0059 |
| Description | A high-purity ammonium sulfate preparation for the selective precipitation and fractionation of proteins from complex biological mixtures. This reagent enables the enrichment of immunoglobulins and other proteins based on their differential solubility. |
| Intended Use | Selective precipitation of proteins, particularly immunoglobulins, from serum, ascites fluid, and cell culture supernatants as a primary purification step. |
| Principle / Technology | Ammonium sulfate increases the ionic strength of protein solutions, reducing the effective solvation layer around proteins and promoting hydrophobic aggregation, leading to selective precipitation at specific salt saturation levels. |
| Detection Method | Precipitation followed by centrifugation; protein pellet reconstitution in appropriate buffer |
| Sample Type | Serum, plasma, ascites fluid, cell culture supernatant, tissue homogenate extracts |
| Performance Range / Specifications | Optimal precipitation for most serum proteins occurs at 40–50% ammonium sulfate saturation; immunoglobulins typically precipitate at 33–40% saturation |
| Sensitivity / LOD | Recovers proteins at concentrations as low as 10 μg/mL from starting material |
| Specificity | Broad precipitation of globular proteins; does not distinguish between specific proteins; selectivity achieved by stepwise saturation adjustments |
| Reaction Conditions / Protocol | Slowly add ammonium sulfate to protein solution with gentle stirring at 4°C, allow to equilibrate for 30–60 minutes, centrifuge at 10,000g for 20 minutes, collect precipitate or supernatant as needed |
| Components / Formulation | Ultra-pure ammonium sulfate (≥99.0% purity), pre-weighed in 500g container; protein precipitation guide with saturation table included |
| Storage Conditions | Room temperature in tightly sealed container; protect from moisture |
| Shelf Life | 36 months from date of manufacture |
| Package Specifications | 500 g |
| Product Form | White crystalline powder |
| Quality Control | Heavy metal content <5 ppm; iron content <3 ppm; tested for absence of proteolytic activity; pH of 1 M solution verified (5.0–6.0) |
| Key Features | Ultra-high purity grade suitable for sensitive enzymatic and immunological applications; pre-weighed format reduces handling time; detailed saturation table simplifies protocol design |
For research use only, not for clinical use.
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