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| Product Name | Amicon Ultra Centrifugal Filter Unit (10K MWCO) |
| Catalog No. | PTR-HMM-0073 |
| Description | A centrifugal ultrafiltration device with a low-binding Ultracel regenerated cellulose membrane and 10,000 Dalton molecular weight cut-off for rapid concentration, desalting, and buffer exchange of protein and nucleic acid samples. |
| Intended Use | Concentration, desalting, and buffer exchange of dilute protein solutions in volumes of 0.5–15 mL using standard benchtop centrifuges. |
| Principle / Technology | Centrifugal force drives solvent and low-molecular-weight solutes through the Ultracel membrane while retaining macromolecules above the specified molecular weight cut-off in the sample reservoir. |
| Detection Method | Concentrated sample recovered by reverse-spin or pipetting from the sample reservoir |
| Sample Type | Purified proteins, antibodies, enzymes, peptides, nucleic acids in aqueous buffer solutions |
| Performance Range / Specifications | Concentration factor: up to 30× in a single centrifugation step; sample volume processed: 0.5–15 mL depending on device size; concentration rate: typical IgG concentration from 1 mg/mL to 20 mg/mL achieved in 10–15 minutes at 4,000g |
| Sensitivity / LOD | Protein recovery >90% when membrane is properly pre-rinsed and samples contain at least 10 μg total protein |
| Specificity | Retention of molecules >10 kDa; typical buffer salt removal >95% in a single concentration-dilution cycle |
| Reaction Conditions / Protocol | Pre-rinse filter with water or buffer, add sample to the device, centrifuge at 4,000–5,000g until the desired concentration is reached, recover concentrated sample from the reservoir by pipetting or reverse-spin centrifugation |
| Components / Formulation | Centrifugal filter devices pre-sterilized and individually packaged; non-sterile option also available; compatible with fixed-angle and swinging-bucket rotors receiving 50 mL conical tubes |
| Storage Conditions | Room temperature; devices are for single use and should not be reused |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 8 devices per package (0.5 mL capacity) or 8 devices per package (4 mL capacity) or 8 devices per package (15 mL capacity) |
| Product Form | Single-use centrifugal ultrafiltration device |
| Quality Control | Each lot tested for molecular weight cut-off accuracy, membrane integrity, and protein recovery using standardized protein solutions |
| Key Features | Low-binding Ultracel membrane minimizes non-specific protein adsorption for high recovery; vertical membrane design reduces concentration polarization and maintains fast flow rates; reverse-spin recovery maximizes concentrated sample retrieval |
For research use only, not for clinical use.
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