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| Product Name | Alcian Blue-PAS Combined Staining Kit |
| Catalog No. | ATRSA-0020 |
| Description | A dual staining kit combining Alcian Blue at pH 2.5 for acid mucopolysaccharide detection with the Periodic Acid-Schiff reaction for neutral mucopolysaccharide and glycogen visualization, enabling comprehensive carbohydrate histochemistry on tissue sections. |
| Intended Use | Simultaneous visualization of acidic mucins (stained blue by Alcian Blue) and neutral mucins/glycogen (stained magenta by PAS reaction) in formalin-fixed paraffin-embedded tissue sections for pathological evaluation. |
| Principle / Technology | Alcian Blue at pH 2.5 electrostatically binds to carboxylated and sulfated acid mucopolysaccharides; periodic acid oxidizes vicinal diols in neutral polysaccharides to aldehydes, which react with Schiff reagent to produce a magenta-colored complex. |
| Detection Method | Bright-field light microscopy; acidic mucins appear blue, neutral mucins and glycogen appear magenta, mixed mucins show purple coloration |
| Sample Type | Formalin-fixed paraffin-embedded tissue sections (4–5 μm thickness) on positively charged glass slides |
| Performance Range / Specifications | Staining protocol: approximately 45 minutes total; applicable to a wide variety of tissue types including gastrointestinal, respiratory, and genitourinary tissues |
| Sensitivity / LOD | Detection of mucin subtypes at histochemically relevant concentrations; Alcian Blue detects acidic mucins at concentrations commonly found in goblet cells and glandular epithelium |
| Specificity | Alcian Blue (pH 2.5) stains sulfated and carboxylated mucins blue; PAS stains neutral mucins, glycogen, and basement membrane magenta; dual staining allows discrimination of mixed mucin populations |
| Reaction Conditions / Protocol | Deparaffinize and hydrate sections, stain with Alcian Blue solution (pH 2.5) for 30 minutes, rinse, oxidize with 1% periodic acid for 5 minutes, rinse, treat with Schiff reagent for 10–15 minutes, rinse in lukewarm water, counterstain with hematoxylin if desired, dehydrate, clear, and mount |
| Components / Formulation | Alcian Blue staining solution (pH 2.5), 1% periodic acid solution, Schiff reagent, Mayer's hematoxylin counterstain, detailed protocol |
| Storage Conditions | 2–8°C for Schiff reagent (protect from light); Alcian Blue solution at room temperature |
| Shelf Life | 12 months for Schiff reagent; 24 months for Alcian Blue solution |
| Package Specifications | Sufficient for 100–200 tissue sections |
| Product Form | Ready-to-use liquid reagents |
| Quality Control | Each lot tested on control tissue sections containing known quantities of acidic and neutral mucins (gastrointestinal tissue, respiratory epithelium); staining intensity and specificity verified by a certified histotechnologist |
| Key Features | Dual detection of mucin subtypes on a single tissue section; the combined blue-magenta staining provides clear visual discrimination for diagnostic pathology; widely used in the characterization of mucin-producing adenocarcinomas |
For research use only, not for clinical use.
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