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| Product Name | Albumin Standard (2 mg/mL BSA in PBS) for Protein Assays |
| Catalog No. | PTR-HMM-0079 |
| Description | A precisely formulated bovine serum albumin standard solution at 2 mg/mL in phosphate-buffered saline, intended for the generation of protein assay standard curves in BCA, Bradford, and Lowry protein quantitation methods. |
| Intended Use | Calibration of protein assay standard curves for accurate concentration determination of unknown protein samples. |
| Principle / Technology | BSA serves as a reference protein with well-characterized behavior in colorimetric protein assays; the absorbance or color development produced by known BSA concentrations is used to construct a standard curve from which unknown sample concentrations are interpolated. |
| Detection Method | Standards are used to generate calibration curves; unknown samples are quantified by interpolation from the standard curve |
| Sample Type | Standards themselves are not biological samples; they are reference materials for assay calibration |
| Performance Range / Specifications | 2 mg/mL stock solution; typical standard curve working range: 25–2000 μg/mL for BCA assay, 100–1500 μg/mL for Bradford assay |
| Sensitivity / LOD | 2 mg/mL ± 2% assigned concentration verified by UV absorbance at 280 nm using the BSA extinction coefficient E1% = 6.6 |
| Specificity | The standard is BSA; for optimal accuracy when quantifying non-BSA proteins, a correction factor or a matched protein standard should be employed |
| Reaction Conditions / Protocol | Dilute the 2 mg/mL stock to prepare a series of standards at appropriate concentrations for the specific protein assay being used; include a blank (0 μg/mL) for zero calibration |
| Components / Formulation | Bovine serum albumin at 2 mg/mL in 0.9% NaCl, 0.05% sodium azide preservative, provided in 5 x 2 mL vials |
| Storage Conditions | 2–8°C; after opening, the standard solution is stable for 6 months when stored at 2–8°C |
| Shelf Life | 24 months from date of manufacture |
| Package Specifications | 5 x 2 mL vials (10 mL total) |
| Product Form | Ready-to-use liquid standard solution, clear and colorless to pale yellow |
| Quality Control | BSA purity >98% by SDS-PAGE; endotoxin <1.0 EU/mg; concentration accuracy verified by amino acid analysis; standard curve linearity confirmed with BCA and Bradford assays |
| Key Features | Ready-to-use format eliminates weighing and dissolution errors; long opened-vial stability reduces waste; ampoule or screw-cap vials minimize contamination risk |
For research use only, not for clinical use.
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