| Product Name | Albumin Content Assay Reagent-Bromocresol Green Colorimetry (Microplate Reader) |
| Catalog No. | PTR-HMM-0054 |
| Description | Albumin is an important plasma protein primarily synthesized and secreted by the liver into the bloodstream. It possesses multiple vital functions and physiological roles, and can bind with various nutrients, hormones, and medications to maintain bodily nutrition and plasma osmotic pressure, while also playing a significant role in immune regulation and antioxidant functions. |
| Application | Albumin carries a positive charge in an acidic environment. In the presence of nonionic surfactants, it can bind with the negatively charged dye bromocresol green (BCG) to form a blue-green complex. The product has a characteristic absorption peak at 630 nm, and the concentration of albumin can be quantitatively determined by measuring changes in absorbance. |
| Applicable Instruments | Microplate Reader |
| Number of Testable Samples | 96 Samples |
| Matching | 96-well plate |
| Detection Time | 4 h (96 Samples) |
| Detection Method | Bromocresol Green Colorimetry |
| Spectral Parameters | 630 nm |
| Signal Response | Incremental |
| Notes | If the ∆A measurement is greater than 0.8, it is recommended to dilute the sample with the extraction solution before testing; If the ∆A measurement is less than 0.01 or the A measurement is close to the A blank, it is recommended to increase the sample volume appropriately before conducting the measurement, and make corresponding adjustments during calculation. If the sample becomes turbid after adding the color developer, it is recommended to dilute the sample appropriately with the extraction solution before conducting the measurement, and make corresponding adjustments during calculation. |
For research use only, not for clinical use.
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