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| Product Name | Agarose LE, Molecular Biology Grade |
| Catalog No. | NATR-HMM-0139 |
| Description | A highly purified low-electroendosmosis (LE) agarose powder specifically formulated for the electrophoretic separation of nucleic acids. This molecular biology grade agarose produces clear, strong gels with minimal background fluorescence and consistent electrophoretic mobility. |
| Intended Use | Preparation of agarose gels for the analytical and preparative separation of DNA fragments from 100 bp to 25 kb by submarine gel electrophoresis in TAE or TBE buffer for PCR product analysis, restriction digestion screening, nucleic acid purification, and Northern/Southern blotting gel preparation. |
| Principle / Technology | Agarose is a linear polysaccharide purified from red seaweed (primarily Gracilaria and Gelidium species). When the powder is dissolved in boiling electrophoresis buffer and cooled, it forms a three-dimensional gel matrix with pore sizes determined by the agarose concentration. DNA molecules migrate through the pores at rates inversely proportional to the log10 of their molecular weight under an applied electric field. |
| Detection Method | Separation of DNA fragments visualized by UV transillumination after ethidium bromide, SYBR Safe, SYBR Green, GelRed, or other fluorescent intercalating dye staining; gel documentation by CCD or CMOS camera imaging systems |
| Sample Type | Double-stranded DNA (PCR products, restriction digests, plasmid DNA, genomic DNA); RNA samples in denaturing formaldehyde gels; compatible with TAE (Tris-Acetate-EDTA) and TBE (Tris-Borate-EDTA) electrophoresis buffer systems |
| Performance Range / Specifications | Gel concentration range: 0.5–4.0% (w/v) recommended; effective separation range: 0.5% gel (1–25 kb), 1.0% (0.5–7 kb), 1.5% (0.2–3 kb), 2.0% (0.1–2 kb); gel strength: >1,200 g/cm² (1% gel); gelling temperature: 34–38°C (1.5% gel); melting temperature: 85–90°C (1.5% gel); sulfate content: ≤0.15%; moisture: ≤10% |
| Sensitivity / LOD | Distinguishes DNA fragments differing by 10% in molecular weight (>500 bp); 50 ng DNA per band detectable by ethidium bromide staining (typical); as little as 2 ng detectable with high-sensitivity stains |
| Specificity | Consistent electrophoretic mobility (Rf) across different lots (CV <5% for DNA standards); low DNA/RNA binding (negligible nucleic acid retention in gel); no detectable DNase or RNase activity |
| Reaction Conditions / Protocol | Weigh appropriate mass of agarose powder, suspend in electrophoresis buffer (TAE or TBE), heat in microwave or boiling water bath until completely dissolved with no visible particles, cool to 50–60°C, add ethidium bromide or other stain if desired, pour into gel casting tray with comb in place, allow to solidify at room temperature for 30–45 minutes, carefully remove comb, place gel in electrophoresis tank and cover with running buffer; run at 5–8 V/cm for appropriate time |
| Components / Formulation | Purified agarose (≥98%), low electroendosmosis (-mr value: 0.10–0.15), DNase/RNase-free, in a moisture-resistant sealed container with desiccant pack |
| Storage Conditions | Room temperature (15–25°C) in tightly sealed container; protect from moisture (hygroscopic); store away from strong oxidizing agents; avoid exposure to direct sunlight and temperature extremes (>30°C) |
| Shelf Life | 60 months from date of manufacture in unopened container at recommended storage conditions |
| Package Specifications | 25 g, 100 g, 500 g, 1 kg, and bulk 5 kg packaging options |
| Product Form | White to off-white free-flowing powder |
| Quality Control | Each lot tested: gel strength >1,200 g/cm² (1% gel); electroendosmosis (-mr) 0.10–0.15; sulfate content ≤0.15%; DNase and RNase negative (functional assay); DNA resolution verified by separation of 100 bp ladder with clear resolution of all bands; gelling temperature within specification; no PCR inhibition when gel-purified DNA used as template |
| Key Features | Ultra-low electroendosmosis ensures sharp, undistorted DNA bands; consistent lot-to-lot performance for reproducible results; wide separation range with different gel percentages; low background fluorescence with all common DNA stains; molecular biology certified for enzyme compatibility with gel extraction workflows |
For research use only, not for clinical use.
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