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| Product Name | ADHP (10-Acetyl-3,7-dihydroxyphenoxazine) Fluorogenic Peroxidase Substrate |
| Catalog No. | RESK-0563 |
| Description | ADHP (10-acetyl-3,7-dihydroxyphenoxazine) is a peroxidase substrate suitable for both absorbance and fluorescence detection and is widely used in fluorometric analysis. It is a highly sensitive probe for hydrogen peroxide and peroxidases: in the presence of peroxidases such as horseradish peroxidase (HRP) or myeloperoxidase (MPO), ADHP reacts with H2O2 in a 1:1 stoichiometry to produce the intensely red-fluorescent compound resorufin. Resorufin has excitation and emission maxima of 571 nm and 585 nm, respectively, and shows strong visible-light absorption at its excitation wavelength, allowing absorbance measurement at 570 nm. ADHP is among the most sensitive and stable fluorescent probes known for HRP and H2O2 detection. Compared with other commercial HRP substrates such as dihydrofluorescein and dihydrorhodamine, ADHP exhibits lower background fluorescence and is less affected by air oxidation. ADHP-based H2O2 detection is also more sensitive than the commonly used scopoletin method. Because H2O2 is generated in many enzyme-catalyzed redox reactions, ADHP can be coupled with enzymatic reactions to assay numerous oxidases, related enzymes, their substrates, and cofactors, including glucose, acetylcholine, cholesterol, L-glutamate, and amino acids. |
| Intended Use | For fluorometric or colorimetric detection of HRP and other peroxidases in immunological assays, quantification of total H2O2, and coupled assays of H2O2-generating oxidases and their substrates (e.g., glucose, acetylcholine, cholesterol, L-glutamate, and amino acids). |
| Principle / Technology | In the presence of a peroxidase (HRP, MPO), ADHP reacts with H2O2 in a 1:1 stoichiometry to generate the strongly red-fluorescent product resorufin (Ex/Em 571/585 nm), which can be measured by fluorescence or by absorbance at 570 nm. |
| Detection Method | Fluorescence detection (Ex 571 nm, Em 585 nm) or absorbance measurement at 570 nm. |
| Sample Type | Samples containing peroxidases (e.g., HRP, MPO), hydrogen peroxide, or H2O2-generating enzymatic reactions. |
| Performance Range / Specifications | Synonym: 10-acetyl-3,7-dihydroxyphenoxazine Reaction stoichiometry: 1:1 with H2O2 in the presence of peroxidase Product: resorufin, Ex/Em 571/585 nm; absorbance readable at 570 nm |
| Sensitivity / LOD | Among the most sensitive and stable fluorescent probes known for HRP and H2O2 detection; ADHP-based H2O2 detection is more sensitive than the scopoletin method. |
| Specificity | Reacts with H2O2 in a 1:1 stoichiometry in the presence of peroxidases such as HRP and MPO; lower background fluorescence and less air-oxidation interference than dihydrofluorescein- or dihydrorhodamine-based substrates. |
| Components / Formulation | Single-component reagent. |
| Storage Conditions | Store at -15 to -25 °C. |
| Shelf Life | 2 years when stored at -15 to -25 °C. |
| Package Specifications | 5 mg; 25 mg; 100 mg |
| Shipping Conditions | Shipped with ice packs. |
| Expiration Date / Stability | Stable for 2 years at -15 to -25 °C. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Suitable for fluorescence and absorbance readout; can be coupled with H2O2-generating enzymatic reactions to assay oxidases, related enzymes, substrates, and cofactors such as glucose, acetylcholine, cholesterol, L-glutamate, and amino acids. |
For research use only, not for clinical use.
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