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| Product Name | 7-AAD Viability Staining Solution |
| Catalog No. | RESK-0550 |
| Description | 7-Aminoactinomycin D (7-AAD) is a nucleic acid dye that emits strong fluorescence upon binding to DNA. Its fluorescence properties are similar to those of propidium iodide (PI): it can be excited by the 488 nm argon-ion laser, but its emission spectrum is narrower and shifted to longer wavelengths, causing less interference with other detection channels. It is an ideal substitute for PI in multicolor fluorescence analysis and can be combined with various dyes excited at 488 nm, such as FITC and PE. 7-AAD is a membrane-impermeant fluorescent dye. It cannot cross the cell membrane of live cells, but it penetrates membrane-compromised cells, such as late apoptotic or necrotic cells, and binds to their DNA. It can therefore be used to distinguish viable early-stage cells from necrotic or late apoptotic cells and is widely used in flow cytometry. |
| Intended Use | Cell viability staining for distinguishing viable cells from necrotic or late apoptotic cells, widely used in multicolor flow cytometric analysis. |
| Principle / Technology | 7-AAD is a membrane-impermeant nucleic acid dye. It is excluded by live cells with intact membranes but enters membrane-compromised late apoptotic or necrotic cells and binds DNA, producing strong fluorescence that can be excited by the 488 nm argon-ion laser. |
| Detection Method | Flow cytometry (FL3 channel); maximum excitation at 546 nm and maximum emission at 647 nm; excitable by the 488 nm argon-ion laser. |
| Sample Type | Cell suspensions; approximately 1 x 10^5 cells per test. |
| Performance Range / Specifications | Ready-to-use 7-AAD staining solution for direct cell staining. Maximum excitation wavelength: 546 nm Maximum emission wavelength: 647 nm Dye concentration: 10 μg/mL Sufficient for 150 tests at 10 uL per test (with 1 x 10^5 cells). |
| Specificity | Membrane-impermeant: stains only cells with compromised membranes (necrotic or late apoptotic cells) and is excluded by viable cells. |
| Reaction Conditions / Protocol | Ready-to-use solution; add directly to the cell sample for staining. Use 10 uL per test containing approximately 1 x 10^5 cells. |
| Components / Formulation | Ready-to-use 7-AAD staining solution at 10 μg/mL. |
| Storage Conditions | Store at 4 °C, dry and protected from light. |
| Shelf Life | 6 months when stored as recommended. |
| Package Specifications | 150 tests |
| Product Form | Ready-to-use liquid solution. |
| Concentration | 10 μg/mL |
| Expiration Date / Stability | Stable for 6 months when stored at 4 °C, dry and protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Can be combined in multicolor fluorescence analysis with various dyes excited at 488 nm, such as FITC and PE; its narrower, longer-wavelength emission causes less interference with other detection channels than PI. |
| Application Notes / Precautions | Detect fluorescence in the FL3 channel of the flow cytometer. Each test (containing 1 x 10^5 cells) uses 10 uL; the product is sufficient for 150 tests. |
For research use only, not for clinical use.
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