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| Product Name | 7-AAD (7-Aminoactinomycin D) |
| Catalog No. | RESK-0532 |
| Description | 7-Aminoactinomycin D (7-AAD) is a nucleic acid dye that emits strong fluorescence upon binding to DNA. Its fluorescence characteristics are similar to those of propidium iodide (PI): it can be excited by the 488 nm argon-ion laser, but its emission spectrum is narrower and shifted to longer wavelengths, causing less interference with other detection channels. 7-AAD is therefore the preferred alternative to PI in multicolor fluorescence analysis and can be combined with a variety of fluorophores excited at 488 nm, such as FITC and PE. In addition, 7-AAD is a membrane-impermeant fluorescent dye. It cannot cross the membrane of living cells but penetrates cells with compromised membranes, such as late apoptotic or necrotic cells, and binds to their DNA. It is therefore used to distinguish viable early-stage cells from necrotic or late apoptotic cells and is widely used in flow cytometry. |
| Intended Use | Nucleic acid staining for discrimination of viable cells from late apoptotic and necrotic cells in flow cytometry and multicolor fluorescence analysis. |
| Principle / Technology | 7-AAD is a membrane-impermeant DNA-binding dye. It is excluded by the intact membrane of living cells but enters membrane-compromised cells (late apoptotic or necrotic cells) and binds DNA, producing strong red fluorescence when excited at 488 nm. |
| Detection Method | Flow cytometry with excitation by the 488 nm argon-ion laser; Ex/Em = 546/647 nm. |
| Sample Type | Cell suspensions containing viable, apoptotic, or necrotic cells. |
| Specificity | Membrane-impermeant: stains only cells with compromised membranes, such as late apoptotic or necrotic cells, while viable early-stage cells are excluded. |
| Components / Formulation | Single-component nucleic acid dye supplied as a solid; soluble in water and DMSO. |
| Storage Conditions | Store dry at -20 °C, protected from light. |
| Shelf Life | 1 year under recommended storage conditions. |
| Package Specifications | 1 mg |
| Product Form | Solid. |
| Molecular Weight | 1270.43 |
| Source / Origin | CAS No. 7240-37-1; molecular formula C62H87N13O16; English synonyms: 7-Aminoactinomycin D; Actinomycin D, 7-amino-. |
| Shipping Conditions | Shipped at room temperature. |
| Expiration Date / Stability | Stable for 1 year when stored dry at -20 °C protected from light. |
| Regulatory / Compliance | For research use only. |
| Compatibility | Excitable by the 488 nm argon-ion laser; narrower and longer-wavelength emission than PI with less interference in other detection channels; compatible with 488 nm-excited fluorophores such as FITC and PE in multicolor fluorescence analysis. |
| Recommended Buffer System | Soluble in water and DMSO. |
| Application Notes / Precautions | Store protected from light. |
For research use only, not for clinical use.
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