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| Product Name | 6-JOE Succinimidyl Ester (6-Carboxy-4',5'-Dichloro-2',7'-Dimethoxyfluorescein, SE) |
| Catalog No. | RERE-0001 |
| Description | 6-JOE, SE is an amine-reactive xanthene dye whose absorption and emission closely resemble rhodamine 6G, which makes it a natural partner in multi-dye panels for automated DNA sequencing. As the succinimidyl ester it couples directly to amines, and the main documented applications are sequencing chemistry and nucleotide labelling. |
| Intended Use | Amine-reactive fluorescent label for automated DNA sequencing workflows and nucleotide labelling. |
| Principle / Technology | The succinimidyl ester reacts with primary amines to form stable amide linkages, attaching the 6-JOE fluorophore to the labelled molecule. |
| Detection Method | Fluorescence detection with excitation/emission of 529/555 nm. |
| Sample Type | Orange solid, supplied as purified free acid ester. |
| Performance Range / Specifications | CAS 113394-23-3; molecular formula C27H17Cl2NO11; molecular weight 602.33 g/mol; appearance orange solid; purity ≥95%; Ex/Em 529/555 nm. |
| Specificity | Spectrally similar to rhodamine 6G; designed for amine labelling rather than thiol- or carboxyl-specific chemistry. |
| Reaction Conditions / Protocol | Dissolve in anhydrous DMSO or a similar aprotic solvent shortly before use, react with the amine-bearing substrate under the chosen buffer conditions, then purify the conjugate. |
| Components / Formulation | One vial of dye. Solvents, buffers and conjugation reagents are not included. |
| Storage Conditions | Store at -25 to -15 °C, protected from light. |
| Shelf Life | 12 months under the recommended storage conditions. |
| Package Specifications | 5 mg or 50 mg |
| Product Form | Orange solid. |
| Quality Control | Released at purity ≥95% against the documented specification. |
| Key Features | - Spectrally matched to rhodamine 6G for multi-dye panels - Purity released at ≥95% - Amine-reactive ester form couplings directly to targets - Two pack sizes, 5 mg and 50 mg - One-year shelf life at -25 to -15 °C protected from light |
| Purity | ≥95% |
| Activity / Unit Definition | Labeling performance is defined by its spectral properties and coupling yield with amines rather than by a unit definition. |
| Molecular Weight | CAS 113394-23-3; molecular formula C27H17Cl2NO11; molecular weight 602.33 g/mol. |
| Source / Origin | Synthetic small-molecule fluorophore, supplied as a purified solid. |
| pH Range / Optimal pH | Does not apply; pH is not a meaningful specification for this product type. |
| Expiration Date / Stability | Stable through the stated shelf life when kept protected from light at the recommended storage temperature. |
| Regulatory / Compliance | Restricted to research applications. |
| Compatibility | Compatible with standard fluorescence instrumentation - microscopes, plate readers, flow cytometers or imaging systems as appropriate to the format. |
| Recommended Buffer System | No buffer is supplied; prepare anhydrous stock solutions immediately before labelling. |
| Application Notes / Precautions | Protect from light at all times, both in storage and during labelling, and avoid repeated warming cycles. Prepare stock solutions fresh where possible and confirm the instrument filter set before starting an experiment. |
| Batch-to-Batch Consistency | Each production lot is released against fixed acceptance criteria, keeping performance consistent between orders. |
| Model Type | Amine-reactive fluorescent dye (succinimidyl ester) |
| Starting Material / Tissue Site | Synthesised 6-JOE fluorophore, esterified as the succinimidyl ester |
| Delivery Standard | Pack sizes 5 mg and 50 mg |
| Turnaround Time | No turnaround figure is published; shipping is arranged when the order is confirmed. |
| Readouts / Downstream Assays | Excitation/emission 529/555 nm on a suitable fluorescence channel. |
For research use only, not for clinical use.
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